1 Abbreviations used: [methyl-C]AdoMet, S-adenosyl-L-[methyl-C]methionine; [methyl-H]AdoMet, S-adenosyl-L-[methyl-H]methionine; aDMA, asymmetric x-N,N-dimethylarginine; AdoMet, S-adenosyl-L-methionine; AdoHcy, S-adenosylL-homocysteine; CID, collision-induced dissociation; DMA, dimethylarginine; DTT, dithiothreitol; ESI, electrospray ionization; ETD, electron-transfer dissociation; FPLC, fast pe...

It is observed that some venom may have an anticancer effect. Hemiscorpius lepturus is a most dangerous scorpion in Iran. Heminecrolysin is the most toxic fraction of Hemiscorpius lepturus venom and it is responsible for hemolytic effects of the venom. In this research, we purified heminecrolysin by FPLC, and HPLC methods and used MTT assay in prostatic cancer cell line (PC3 cells). We observed...

Endogenous factors preferentially inhibiting T-lymphocyte proliferation were prepared from the acetone precipitate of a 60% ethanol extract from calf thymus and their biochemical properties examined. By ultrafiltration the strongest lymphocyte-selective inhibition was found in the molecular mass range between 1 and 5 kDa. Fast protein liquid chromatography (FPLC) of this fraction on an anion ex...

Cytosolic extracts of boar sperm contain a soluble phospholipase C (PLC) activity that induces Ca2+ release in sea-urchin (Lytechinus pictus) egg homogenates and an uncharacterized protein factor that causes Ca2+ oscillations when injected into mammalian eggs. In the present study we fractionated boar sperm extracts on three different FPLC chromatographic columns and found that the fractions th...

In this preliminary report we show that a 29 kDa surface protein of Listeria monocytogenes EGD removed from cells with 4 M LiCl has peptidoglycan (murein) hydrolyzing activity, as revealed by zymographic analysis using Bacillus subtilis murein and heat-killed Micrococcus luteus cells casted in the gel. Following two-dimensional electrophoresis, the protein was electroblotted to PVDF membrane an...

THE PURPOSE of this study was to purify and characterize hepatic stimulatory substance (HSS), a protein found in the liver that has been reported to stimulate hepatic DNA synthesis and hepatocyte replication and that was previously identified by LaBrecque et al l -3 and Francavilla et al in the rat liver,4,5 as well as by Starzl et al in the canine liver.6,7 HSS has been partially purified by s...

Abbreviations: 17 severe acute respiratory syndrome, SARS; SARS coronavirus, SARS-CoV; main protease, M; 18 wild-type, WT; ethylene glycolbis succinimidylsuccinate, EGS; 1,4-Dithiothreitol, DTT; 19 2,2-dimethyl-2-silapentanesulfonic acid , DSS; mass spectrometry, MS; fast protein liquid 20 chromatography system, FPLC; nuclear magnetic resonance, NMR; heteronuclear 21 single-quantum coherence, H...

Methods The proteins 2S, 11S and oleosins were purified from a raw sunflower seed extract by FPLC/HPLC and identified by specific antibodies and peptide mass fingerprinting. We tested the immunological recognition of these proteins by basophil activation test (BAT). Four concentrations (1, 0.2, 0.1, 0.02 ìg/ml) of each protein and the sunflower roasted extract were used. Ten patients were selec...

.................................................................................................. 1 Zusammenfassung ..................................................................................... 2 2. West Nile Virus Introduction ............................................................... 4 1.1. Virology ...................................................................................

Curcumin, which has been used in ancient Indian Ayurvedic system of medicine, has recently been shown to have anti-cancer activity. However, its poor solubility in water has restricted its development as an effective anti-cancer agent for clinical use. We report the synthesis of a well-defined water soluble Bovine Serum Albumin (BSA)-curcumin conjugate. The conjugate was characterized via Gel e...