Genes encoding homologs of the gp91(phox) subunit of the plasma membrane NADPH oxidase complex have been identified in plants and are hypothesized to be a source of reactive oxygen species during defense responses. However, the direct involvement of the gene products in superoxide (O(2)(-)) production has yet to be shown. A novel activity gel assay based on protein fractionation in native or so...

Genes encoding homologs of the gp91 subunit of the plasma membrane NADPH oxidase complex have been identified in plants and are hypothesized to be a source of reactive oxygen species during defense responses. However, the direct involvement of the gene products in superoxide (O2 ) production has yet to be shown. A novel activity gel assay based on protein fractionation in native or sodium dodec...

In the present study, five naphthalene-based azo dyes were obtained using classical azo coupling reactions. The structure of the dyes was characterized and confirmed by the UV-vis, FT-IR, 1H NMR and 13C NMR analyses. The solvatochromic behavior of the dyes was studied in a set of 10 solvents with different polarity and considerable results were obtained. The dyes were applied as disperse dyes t...

Cytotoxicity presents one of the required criteria in biological evaluation medical devices. In this study, semi-direct contact test was used to evaluate potential cytotoxicity hydrogel wound dressings compared conventional extract test. Three types sheets were fabricated from poly(vinyl alcohol) (PVA) by gamma irradiation: Bare sheets, silver (Ag)-coated and Aloe vera (AV)-coated sheets. test,...

This new assay procedure for diaphorase eliminates problems of high blank rates and nonlinear kinetics associated with other methods. The dye thiazolyl blue tetrazolium bromide is reduced in the presence of NADH and diaphorase to yield a colored formazan, which as maximum absorbance at 560 nm.

Labeling of metastatic tumors can aid in their staging and resection of cancer. Near infrared (NIR) dyes have been used in the clinic for tumor labeling. However, there can be a nonspecific uptake of dye by the liver, lungs, and lymph nodes, which hinders detection of metastasis. In order to overcome these problems, we have used two NIR dyes (DyLight 650 and 750) conjugated to a chimeric anti-c...

Seaweeds have caused an emerging interest in the biomedical area, mainly due to their contents of bioactive substances which show great potential as anti-inflammatory, anti-microbial, anti-viral and anti-tumoral drugs. Despite the diversity in quality and quantity of the Mediterranean Tunisian coast flora, with its large contains of marine organisms and seaweeds, most of them have not yet been ...

The use of reactive dyes has increased rapidly due to outstanding properties, growing consumption of cellulosic fibers, and decrease in the production of other application classes of dyes for cellulosics because of cost and health hazards. However, reactive dyes cause the greatest effluent problems for the dye industry. This study explored reusing these dyes in which the residual dyeings were d...

objective: the aim of the present study was to evaluate the efficacy of two caries detector dyes in the diagnosis of dental caries. materials and methods: twenty extracted human posterior teeth without pulpal exposure were sectioned mesiodistally through the center of the lesions using a water-cooled disk. the tooth halves were randomly divided into two groups and treated with caries detector (...