OBJECTIVE To evaluate the efficiency of L-glutamic acid to prevent calcification of glutaraldehyde bovine pericardium implanted in rats' subcutaneous tissues. METHODS Fifty four Wistar rats were divided in six groups according to the type of the bovine pericardium implanted. At first, all pericardia were initially cross-linked with 0.5% glutaraldehyd (GDA) fixative for 72 h. In Group I, after...

This study evaluates the structural organization of the cytoskeleton within unactivated, discoid platelets. Previously, such studies have been difficult to interpret because of the ease with which platelets are stimulated, the sensitivity of actin filaments to cell extraction buffers, and the general problem of preserving actin filaments with conventional fixatives, compounded by the density of...

If fixation is not adequate, the other processes that follow such as dehydration, clearing, infiltration, embedding, microtomy and staining, will also be inadequate. A poorly processed tissue make it difficult for Pathologist/Histoscientist to render a proper diagnosis. This article aimed at elucidating more on fixatives process in histopathology. The information presented this review was gathe...

Abstract Sperm cells are variable both within and among species. To be able to accurately measure sperm understand their function, it is important that preserved in a manner maintains structural integrity. Formalin widely used fixative storage medium for cells, but few studies have examined the effect of fixation long-term on morphological Ethanol also common agent tissue samples, here we exami...

Fixation is a critical step in the preparation of tissues for histopathology. The objective of this study was to investigate the effects of different fixatives vs formalin on proteins and DNA, and to evaluate alternative fixation for morphological diagnosis and nucleic acid preservation for molecular methods. Forty tissues were fixed for 24 h with six different fixatives: the gold standard fixa...

Tissue fixation in phosphate buffered formalin (PBF) remains the standard procedure in histopathology, since it results in an optimal structural, antigenic and molecular preservation that justifies the pivotal role presently played by diagnoses on PBF-fixed tissues in precision medicine. However, toxicity of formaldehyde causes an environmental concern and may demand substitution of this reagen...

On average, 15 per cent of the total haemoglobin present in the blood of the newt Triturus cristatus was extracted during 45 minutes of fixation in Palade-Caulfield fixative. This extraction was reduced with fixatives buffered at pH 6.2 instead of pH 7.4. The addition of Ca(++) ions to a final concentration of 0.01 M in the fixative completely suppressed haemoglobin extraction. The effect of th...