Glycyrrhiza glabra residue (GGR) was efficiently subjected to concentrated phosphoric acid (PA) pretreatment with/without surfactant assistance, and promising results were obtained following separate enzymatic hydrolysis and fermentation (SHF) of the biomass. Pretreatment was carried out using 85 % PA either at 50 or 85 °C with 12.5 % solid loading for 30 min. In parallel experiments, ...

The growing interest in Green Analytical Chemistry (GAC) principles through the replacement of polluting analytical procedures with greener ones, has encouraged us to develop an eco-friendly stability-indicating HPLC diode array detection method (HPLC-DAD) for simultaneous determination allopurinol (ALP) and benzbromarone (BNZ). Effective separation was accomplished using Durashell C18 column (...

ABSTRACT: This article provides an overview on Development and Validation of Force degradation study with Stability Indicating Methods (SIMs) for drug substances. Different stress conditions (hydrolysis, oxidation, thermal, photolytic conditions) are applied to drugs compounds during a process known as forced degradation, result, various products created. The major purpose these investigations ...

It is well known, that forced periodic operation possesses the potential for process improvements. Nevertheless, only a small number of applications reported, due to complex realization, limited predictability and high inertia larger units. Nonlinear frequency response (NFR) analysis has proven predict efficiently time-averaged performance reactor effluent streams originating from changes one o...

  The forced degradation study of lidocaine HCl was carried out according to the ICH guideline Q1A (R2). The degradation conditions were assessed to be hydrolysis, oxidation, photolysis and dry heat during 24 h, 48 h and 72 h and then the samples were investigated by GC-FID method and nuclear magnetic resonance (NMR) spectroscopy. According to these results, the degradation products were not o...

The typical archaeal MCM exhibits helicase activity independently in vitro. This study characterizes MCM from the euryarchaeon Picrophilus torridus. While PtMCM hydrolyzes ATP in DNA-independent manner, it displays very poor ability to unwind DNA independently, and then too only under acidic conditions. The protein exists stably in complex with PtGINS in whole cell lysates, interacting directly...

Ice cream mixes were treated with lactase (EC 3.2.1.23) to cause 0 to 83% lactose hydrolysis. Lactose hydrolysis decreased the freezing point from -1.63C in the control (0% hydrolyzed) samples to -1.74C in the 83% hydrolyzed sample (p < 0.05). Firmness decreased from 0.35 J in the control sample to 0.08 J in the 83% hydrolyzed sample. Lactose hydrolyzed samples melted at a faster rate than the ...

The phenomenological model of sulphate anions effect on the nanodispersed titania synthesis during hydrolysis of titanium tetrachloride was studied. It was proposed that both chelating and bridging bidentate complexes formation between sulphate anions and octahedrally coordinated [Ti(OH)h(OH2)6-h](4-h)+ mononers is the determinative factor for anatase phase nucleation.

The aim of this work was to demonstrate the effectiveness of hydrolysis pretreatment of olive mill (OMW) waste before use as a carbon source in biosurfactant production by fermentation. Three hydrolysis methods were assessed: enzymatic hydrolysis, acid pretreatment plus enzymatic hydrolysis, and acid hydrolysis. Fermentation was carried out using two bacterial species: Pseudomonas aeruginosa an...

An adsorption-kinetic model has been proposed to explain -galactosidase stability in a recirculation hollow-fibre bioreactor at different temperature. The kinetic constants of the model and its dependence on temperature have been evaluated. In addition, a study has been made of the influence of lactose and enzyme concentration on lactose hydrolysis with hollow-fibre modules m s a m a ©