نتایج جستجو برای: fmdv

تعداد نتایج: 872  

2015
Kazuki Morioka Katsuhiko Fukai Kazuo Yoshida Rie Kitano Reiko Yamazoe Manabu Yamada Tatsuya Nishi Toru Kanno Xia Jin

We developed a lateral flow strip using monoclonal antibodies (MAbs) which allows for rapid antigen detection and serotyping of foot-and-mouth disease virus (FMDV). This FMDV serotyping strip was able to detect all 7 serotypes and distinguish serotypes O, A, C and Asia1. Its sensitivities ranged from 10(3) to 10(4) of a 50% tissue culture infectious dose of each FMDV stain; this is equal to tho...

Journal: :Acta virologica 1997
B Pattnaik A Sanyal M George C Tosh D Hemadri R Venkataramanan

Eight oligonucleotide primers in 7 different combinations were used to amplify 3D gene sequences of foot-and-mouth disease virus (FMDV) by reverse transcription-polymerase chain reaction (RT-PCR). Six of the primers were designed at this laboratory. All the primer combinations could specifically amplify 3D gene sequences of FMDV serotypes O, A, and C. The largest fragment amplified was of 1,393...

2008
Huichen GUO Shiqi SUN Jiangtao MA Zaixin LIU Xiangtao LIU Qingge XIE

The study was conducted to evaluate the immunogenicity and protective efficacy of a DNA vaccine against foot-and-mouth disease virus (FMDV). Genes encoding the P1, 2A, 3C of FMDV O/China99 and bovine IFN-α were cloned into pcDNA3.1 (+) expression vector under CMV promoter and FMDV IRES control, respectively. The recombinant plasmids were administered to guinea pigs by intramuscular injection wi...

Journal: :Comparative immunology, microbiology and infectious diseases 2002
Esteban Domingo Eric Baranowski Cristina Escarmís Francisco Sobrino

Foot-and-mouth disease virus (FMDV) is an aphthovirus of the family Picornaviridae and the etiological agent of the economically most important animal disease. As a typical picornavirus, FMD virions are nonenveloped particles of icosahedral symmetry and its genome is a single stranded RNA of about 8500 nucleotides and of positive polarity. FMDV RNA is infectious and it replicates via a compleme...

2011
Miriam A Windsor B Veronica Carr Bartomiej Bankowski Debi Gibson Elizabeth Reid Pip Hamblin Simon Gubbins Nicholas Juleff Bryan Charleston

Infection of cattle with foot-and-mouth disease virus (FMDV) results in the development of long-term protective antibody responses. In contrast, inactivated antigen vaccines fail to induce long-term protective immunity. Differences between susceptible species have also been observed during infection with FMDV, with cattle often developing persistent infections whilst pigs develop more severe sy...

Journal: :Revue scientifique et technique 2016
N J Knowles J Wadsworth K Bachanek-Bankowska D P King

Nucleotide sequences of field strains of foot and mouth disease virus (FMDV) contribute to our understanding of the distribution and evolution of viral lineages that circulate in different regions of the world. This paper outlines a practical reversetranscription polymerase chain reaction (RT-PCR) and sequencing strategy that can be used to generate RNA sequences encoding the VP1 (1D) region of...

2018
E L A Howson B Armson N A Lyons E Chepkwony C J Kasanga S Kandusi N Ndusilo W Yamazaki D Gizaw S Cleaveland T Lembo R Rauh W M Nelson B A Wood V Mioulet D P King V L Fowler

Effective control and monitoring of foot-and-mouth disease (FMD) relies upon rapid and accurate disease confirmation. Currently, clinical samples are usually tested in reference laboratories using standardized assays recommended by The World Organisation for Animal Health (OIE). However, the requirements for prompt and serotype-specific diagnosis during FMD outbreaks, and the need to establish ...

2016
Zixiang Zhu Guoqing Wang Fan Yang Weijun Cao Ruoqing Mao Xiaoli Du Xiangle Zhang Chuntian Li Dan Li Keshan Zhang Hongbing Shu Xiangtao Liu Haixue Zheng

The role of retinoic acid-inducible gene I (RIG-I) in foot-and-mouth disease virus (FMDV)-infected cells remains unknown. Here, we showed that RIG-I inhibits FMDV replication in host cells. FMDV infection increased the transcription of RIG-I, while it decreased RIG-I protein expression. A detailed analysis revealed that FMDV leader proteinase (Lpro), as well as 3C proteinase (3Cpro) and 2B prot...

2014
Chun-Xue Zhou Dong Li Ying-Li Chen Zeng-Jun Lu Pu Sun Yi-Mei Cao Hui-Fang Bao Yuan-Fang Fu Ping-Hua Li Xing-Wen Bai Bao-Xia Xie Zai-Xin Liu

BACKGROUND Toll-like receptor (TLR) agonists reportedly have potent antiviral and antitumor activities and may be a new kind of adjuvant for enhancing immune efficacy. Resiquimod (R848) is an imidazoquinoline compound with potent antiviral activity and functions through the TLR7/TLR8 MyD88-dependent signaling pathway. Polyinosinic-polycytidylic acid [poly(I:C)] is a synthetic analog of double-s...

2015
Carla Bravo de Rueda Mart CM de Jong Phaedra L Eblé Aldo Dekker

Foot-and-mouth disease virus (FMDV) infected animals can contaminate the environment with their secretions and excretions. To quantify the contribution of a contaminated environment to the transmission of FMDV, this study used calves that were not vaccinated and calves that were vaccinated 1 week prior to inoculation with the virus in direct and indirect contact experiments. In direct contact e...

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