Site-specific recombinases are being developed as tools for "in vivo" genetic engineering because they can catalyze precise excisions, integrations, inversions, or translocations of DNA between their distinct recognition target sites. Here it is demonstrated that Flp recombinase can effectively mediate site-specific excisional recombination in mouse embryonic stem cells, in differentiating embr...

1.1. This is the first of two papers addressing a Cauchy type inequality for the geometric intersection number between two 1-dimensional submanifolds in a surface. As a consequence, we reestablish some of the basic results in Thurston's theory of measured laminations. In this paper, we consider surfaces with non-empty boundary using ideal triangulations. In the sequel, we establish the inequali...

The FLP protein, a site-specific recombinase encoded by the 2 micron plasmid of yeast, has been purified to near homogeneity from extracts of E. coli cells in which the protein has been expressed. The purification is a three column procedure, the final step employing affinity chromatography. The affinity ligand consists of a DNA polymer with multiple FLP protein binding sites arranged in tandem...

PIN proteins are auxin export carriers that direct intercellular auxin flow and in turn regulate many aspects of plant growth and development including responses to environmental changes. The Arabidopsis R2R3-MYB transcription factor FOUR LIPS (FLP) and its paralogue MYB88 regulate terminal divisions during stomatal development, as well as female reproductive development and stress responses. H...

The logic of single-conclusion (functional) proofs (FLP) is introduced. It combines the veri$cation property of proofs with the single valuedness of proof predicate and describes the operations on proofs induced by modus ponens rule and proof checking. It is proved that FLP is decidable, sound and complete with respect to arithmetical proof interpretations based on single-valued proof predicate...

Binding of a partial proteolytic digest by V8 enzyme of the yeast site-specific recombinase Flp to its target site gives rise to DNA-protein complexes that are smaller than those produced by the full-sized protein. The smallest of these complexes (occupancy of one peptide monomer per site) contains either one of two polypeptides (32 and 28 kDa) of the V8 digestion mixture. The amino termini of ...

Information retrieval (IR) and figurative language processing (FLP) could scarcely be more different in their treatment of language and meaning. IR views language as an open-ended set of mostly stable signs with which texts can be indexed and retrieved, focusing more on a text’s potential relevance than its potential meaning. In contrast, FLP views language as a system of unstable signs that ca...

Horizontal gene transfer has been well documented as a significant feature of genome evolution in prokaryotes [1–3]. The frequency and significance of this process in eukaryotic evolution is much less clear. Confirming that a gene has entered a species by a horizontal route can be difficult, and many previously reported cases of horizontal gene transfer from a prokaryote to a eukaryote have sub...

The answer set semantics presented by Faber et al. (2011) has been widely used to define so called FLP answer sets for different types of logic programs. However, it was recently observed that when being extended from normal to more general classes of logic programs, this approach may produce answer sets with circular justifications that are caused by self-supporting loops. The main reason for ...

The Flp and Cre recombinases are members of the integrase family of tyrosine recombinases. Each protein consists of a 13 kDa NH(2)-terminal domain and a larger COOH-terminal domain that contains the active site of the enzyme. The COOH-terminal domain also contains the major determinants for the binding specificity of the recombinase to its cognate DNA binding site. All family members cleave the...