The use of protein reagents which exhibit sensitivity to changes in molecular environment has provided a means of examining certain properties of enzymes during interaction with their substrates (1). The recently reported 2-hydroxy-5nitrobenzyl bromide provided one such reagent, the absorption spectrum of which showed sensitivity to environmental changes by virtue of the p-nitrophenolic groupin...

BACKGROUND We developed skin prick test (SPT) reagents for common inhalant allergens that reflected the real exposure in Korea. The study aim was to evaluate diagnostic usefulness and allergen potency of our inhalant SPT reagents in comparison with commercial products. METHODS We produced eight common inhalant allergen SPT reagents using total extract (Prolagen): Dermatophagoides farinae, Der...