The role of flagella in the pathogenesis of F4ac+ Enterotoxigenic Escherichia coli (ETEC) mediated neonatal and post-weaning diarrhea (PWD) is not currently understood. We targeted the reference C83902 ETEC strain (O8:H19:F4ac+ LT+ STa+ STb+), to construct isogenic mutants in the fliC (encoding the major flagellin protein), motA (encoding the flagella motor), and faeG (encoding the major subuni...

Here we determined the role of various genomic islands in E. coli K1 interactions with phagocytic A. castellanii and nonphagocytic brain microvascular endothelial cells. The findings revealed that the genomic islands deletion mutants of RS218 related to toxins (peptide toxin, α -hemolysin), adhesins (P fimbriae, F17-like fimbriae, nonfimbrial adhesins, Hek, and hemagglutinin), protein secretion...

Resistance to phagocytosis and epithelial cell binding of Group A streptococci is undoubtedly mediated by surface to surface interactions between host and bacterial cells. Previous ultrastructural studies have demonstrated that the M antigen is associated with surface projections which have been referred to as "fimbriae" (1). The observations were based on ultrastructural studies comparing M-pr...

Enterotoxigenic Escherichia coli (ETEC) are an important cause of post-weaning diarrhea (PWD) in piglets. Porcine-specific ETEC strains possess different fimbrial subtypes of which F4 fimbriae are the most frequently associated with ETEC-induced diarrhea in piglets. These F4 fimbriae are potent oral immunogens that induce protective F4-specific IgA antibody secreting cells at intestinal tissues...

Marginal periodontitis is not a homogeneous disease but is rather influenced by an intricate set of host susceptibility differences as well as diversities in virulence among the harbored organisms. It is likely that clonal heterogeneity of subpopulations with both high and low levels of pathogenicity exists among organisms harbored by individuals with negligible, slight, or even severe periodon...

K99 fimbriae of enterotoxigenic Escherichia coli consist of eight different subunits. A major subunit called fimbrillin forms fimbrial structure and a minor subunit called adhesin localizes at the tip of fimbriae and recognizes host receptor ganglioside. Within this eight gene cluster, fanE and fanF have not yet been sequenced. In this study, fanE and fanF genes were sequenced by analyzing seve...

BACKGROUND Bacterial invasion of host epithelial cells plays an important role in the pathogenesis of periodontitis; however, the exact mechanism of the invasion has not been investigated. METHODS Pocket epithelium biopsies in periodontitis were analysed via scanning and transmission electron microscopy using ultra-histochemical staining with ruthenium red for glycocalyx visualization. RESU...

Fimbrial antigens are very often associated with pathogenic Escherichia coli strains, especially those which cause extraintestinal infections. These fimbriae have the ability to agglutinate erythrocytes {hemagglutination) and can be further grouped according to their receptor specificity. P-fimbriae recognize the 2-D-Gal-{1 -4)-ß-D-Gal region of globotetraosylceramide and trihexosylceramide, wh...

Urinary tract infection with Proteus mirabilis may lead to serious complications, including cystitis, acute pyelonephritis, fever, bacteremia, and death. In addition to the production of hemolysin and the enzyme urease, fimbriae and flagellum-mediated motility have been postulated as virulence factors for this species. We purified mannose-resistant/proteuslike (MR/P) fimbriae and flagella from ...

Enterotoxigenic Escherichia coli (ETEC) is the causative agent of a wide range diseases, which are important cause illness and mortality in piglets. ETEC strains expressing F4 fimbriae frequently associated with post-weaning diarrhea (PWD) lead to great economic losses swine production industry worldwide. The aim this study was establish rapid effective isothermal amplification method for detec...