Lysosome-associated membrane proteins 1 and 2 (LAMP-1 and LAMP-2) are delivered to phagosomes during the maturation process. We used cells from LAMP-deficient mice to analyze the role of these proteins in phagosome maturation. Macrophages from LAMP-1- or LAMP-2-deficient mice displayed normal fusion of lysosomes with phagosomes. Because ablation of both the lamp-1 and lamp-2 genes yields an emb...

Lysosomal membrane glycoprotein (lamp)-1 and lamp-2 are the most abundant glycoproteins within the lysosomal membrane. A small amount of lamp-1 and lamp-2 molecules, however, can be present on the cell surface. We have shown previously that highly metastatic colonic carcinoma L4 cells express more lamp-1 and lamp-2 on the cell surface than low metastatic SP cells (Saitoh, O., Wang, W.-L., Lotan...

Gıda ve çevresel kaynaklarda Salmonella'nın hızlı, güvenilir hassas tespiti, halk sağlığını korumak için çok önemlidir. LAMP yöntemi özel cihazlara deneyimli personele ihtiyaç duymaksızın uygulanabilecek bir yöntem olduğu son yıllarda sıklıkla tercih edilmektedir. LAMP’ın, mikroakışkan cihazlarla kombine edilmesi sınırlı kaynakların durumlarda, hızlı tanı testleri alternatifdir. Salmonella spp....

Bovine viral diarrhea virus (BVDV) is a pathogen that infects cattle, and is globally important. It causes substantial financial losses to the livestock industry. In the current study, a one-step reverse transcriptase-loop-mediated isothermal amplification (RT-LAMP) assay was set up for rapid and efficient detection of BVDV. For this purpose, four primers were designed to recognize six distinct...

background and purpose: salmonella is a group of enterobacterias that cause infectious diseases in human and animals. typhoid, bacteremia, enterocolitis and salmonellosis which are caused by this bacterium are considered major health concerns in developing countries, such as iran. quick, accurate, reliable and early diagnosis is vital to prevent salmonella outbreak. materials and methods: there...

A cDNA encoding the chicken orthologue of dendritic cell-lysosomal associated membrane protein (DC-LAMP)/CD208 was cloned by RT-PCR from RNA isolated from mature chicken bone marrow-derived dendritic cells (chBM-DCs). The cloned chicken DC-LAMP (chDC-LAMP) cDNA consists of 1281 nucleotides encoding an open reading frame of 426 amino acids (aa). Comparison of the deduced aa sequence of DC-LAMP w...

Lysosomes are cellular organelles containing diverse classes of catabolic enzymes that are implicated in diverse cellular processes including phagocytosis, autophagy, lipid transport, and aging. Lysosome-associated membrane proteins (LAMP-1 and LAMP-2) are major glycoproteins important for maintaining lysosomal integrity, pH, and catabolism. LAMP-1 and LAMP-2 are constitutively expressed in Sal...