The yields of the Armstrong and WE strains of lymphocytic choriomeningitis virus in baby hamster kidney (BHK) cells cultivated in either bovine, calf, fetal bovine, or horse serum were investigated. Lines of BHK cells were established in these sera. When the infected cell lines were observed by immunofluorescence, the per cent fluorescing cells for a given virus strain did not vary. However, fo...

To investigate serum requirements for optimal erythropoiesis in vitro, we studied the response of erythroid progenitor cell proliferation in culture to platelet-derived growth factor (PDGF). Human bone marrow cells cultured with platelet-poor plasma-derived serum (PDS) form fewer erythroid colonies than do cells cultured with human whole blood serum or fetal calf serum (P less than 0.05). Treat...

The purpose of the present study was to evaluate the functional and biochemical effects of serum concentrations on hypothermic preservation of cardiac myocytes. Myocytes were isolated from neonatal rat ventricles and cultured for 4 days, and then incubated for 24 hrs at 4 degrees C in media containing 0, 2, 5, 10, and 20% fetal calf serum (FCS). The myocytes were then cultured for an additional...

Abbreviations: BSA: bovine serum albumin CHO: Chinese hamster ovary CL: total plasma clearance Cmax: maximum plasma concentration ELISA: enzyme-linked immunosorbent assay FCS: fetal calf serum ip: intraperitoneal iv: intravenous PBS: phosphate-buffered saline rhGM-CSF: recombinant human granulocyte-macrophage colony-stimulating factor t1/2 α: distribution half-life t1/2 : β terminal half-life T...

isolation of cell free fetal dna (cffdna) from maternal serum usually leads to very low concentrations of dna impeding further resolving through conventional methods of electrophoresis. although several protocols have been described for capillary electrophoresis (ce) of double stranded dna, they usually need using special polymers or coated capillaries which degrade over time. herein, we propos...

Two cell lines with different in vitro growth patterns were established from the pleural fluid of a patient with malignant epithelial pleural mesothelioma. The cell line established in RPMI 1640 supplemented with human AB serum had an epithelial morphology, while the cell line established in fetal calf serum-supplemented medium had a fibroblast-like morphology. Exposure of the fibroblast-like c...

Submit Manuscript | http://medcraveonline.com Abbreviations: LCT: Lambda-Cyhalothrin; LD: Lethal Dose; NOAEL: No-Observed-Adverse-Effect Level; Con A: Concanavalin A; SRBCs: Sheep Red Blood Cells; WBCs: White Blood Cells; Ig: Immunoglobulin; HA: Hemagglutination; QHS: Quantitative Hemolysis of SRBCs; FCS: Fetal Calf Serum; DTH: Delayed-type of Hypersensitivity Response; BMCs: Blood Mononuclear ...

The male contraceptive agent gossypol was found to induce a dose related increase of DNA strand breaks in human fibroblasts in vitro at concentrations of 5 to 40 micrograms/ml. The effect was reduced in the presence of 2% fetal calf serum. A weak but reproducible increase in the SCE frequency was found in human lymphocytes treated for 1 hour in serum-free medium with 0.04 to 4 micrograms/ml of ...

Chymotrypsin enhanced fowlpox virus plaque formation in chick embryo cell cultures. A simplified plaque assay for fowlpox virus is described. Plaques were produced in 3 days when chymotrypsin was included in a serum-free fluid overlay. Plaques were also produced in 5 to 6 days under an agar overlay when a medium containing fetal calf serum was employed. Kinetics of plaque formation were also st...

Significant inhibition of lymphoid tumor development was observed in X-irradiated strain C57BL mice treated with multiple injections of rat antiserum prepared by im munization of Wistar-Furth or (Wistar-Furth-BN) F, rats with histocompatible lymphomas induced by the radiation leukemia virus (RadLV). However, there was also signifi cant inhibition, relative to phosphate-buffered saline con trols...