introduction: standard semen analysis is routinly used to evaluate the male fertility, however some researchers belive that sperm functional test should also be used with standard semen analysis for more precise evaluation of male fertility. the aim of this study was to consider the relation between different nuclear maturity tests and in vitro fertilization, in order to select the nuclear matu...

Background: Failed fertilization post ICSI has been mainly attributed to the sperm’s inability to induce oocyte activation. Phospholipase C Zeta (PLCζ) is considered to be a primary factor for the induction of oocyte activation. The aim of this study was to quantitatively assess the expression of PLCζ in globozoospermic or individuals with previous low or failed fertilization in comparison to f...

a two-year laboratory study on the biology (including self-fertilization) of bulinus truncatus, the intermediate host of schistosoma haematobium, s. bovis and paramphistomum microbotrium in iran, has revealed information on the longevity, reproduction, hatchability rate, hatching time during various months of the year, and growth and mortality rates of this snail. it was found that the reproduc...

BACKGROUND Minimal stimulation in vitro fertilization (mini-in vitro fertilization) is an alternative in vitro fertilization treatment protocol that may reduce ovarian hyperstimulation syndrome, multiple pregnancy rates, and cost while retaining high live birth rates. OBJECTIVE We performed a randomized noninferiority controlled trial with a prespecified border of 10% that compared 1 cycle of...

Standardization of fertilization protocols for sterlet Acipenser ruthenus is crucial for improving reproductive techniques and for conservation purposes. Our objectives were to determine the number of sperm (tested 430,000:1, 43,000:1, 4300:1, 430:1 sperm to egg) required to fertilize eggs and explore how pre-incubation of eggs in freshwater for 0min, 0.5min, 1min, and 10min interacts with diff...

background: human menopausal gonadotropin (hmg) has contributed many improvements to human assisted reproduction. however, effects of hmg on oocyte development and clinical results remain controversial. objective: this study was conducted to investigate the effects of hmg on the zona pellucida of oocytes, as well as clinical results in superovulation treatment. materials and methods: this retro...

Objective. To determine whether CD9 expression on human granulosa cells (GCs) and platelets could predict the success of conventional fertilization of human oocytes during in vitro fertilization (IVF). Methods. Thirty women undergoing IVF for nonmale factor infertility participated. Platelets from venous blood and GCs separated from retrieved oocytes were prepared for immunofluorescence. Flow c...

In this study, fertilization, hatching and deformation rate of the eggs, and larvae obtained from hormone injected (HI) and non-injected broodstock (NHI), were determined. In the experiment, no differences were observed in the groups’ fertilization rates (p>0.05). Hatching rate was found higher in the HI group (p<0.05). During the experiment, malformations such as spinal fluid accumulation, sad...