Fed-batch culture strategy is often used for increasing production of heterologous recombinant proteins in Escherichia coli. This study was initiated to investigate the effects of dissolved oxygen concentration (DOC), complex nitrogen sources and pH control agents on cell growth and intracellular expression of streptokinase (SK) in recombinant E. coli BL21(DE3). Increase in DOC set point from 3...

This paper presents a comparison between batch and three different sets of fed batch fermentations forrhamnolipid production by Pseudomonas aeruginosa. The batch run was performed with 500 ml of culturemedium having the initial glycerol and sodium nitrate concentrations of 30 and 8.3 g/l, respectively. For a fedbatch run with nitrogen source in feed, 250 ml of the nitrogen exc...

To increase cell concentration and the production of alkaline protease, fed-batch cultures with standard medium (1x) or concentrated medium (2x or 5x) as feed were performed. In a fed-batch culture with standard medium as feed, the biomass concentration reached 9.6 g L–1 and the protease activity obtained was 8500 U mL–1 compared to 3000 U mL–1 obtained by growing Teredinobacter turnirae cells ...

This article demonstrates, using simulations, the potential of the S-system formalism for the inference of unknown chemical reaction networks from simple experimental data, such as that typically obtained from laboratory scale reaction vessels. Virtually no prior knowledge of the products and reactants is assumed. S-systems are a power law formalism for the canonical approximate representation ...

In a large number of industrial semi-batch reactor applications, a single reaction takes place in the reactor and the operational objective is to compute the optimal feed rate of reactants and the optimal batch temperature that optimizes an objective function at the end of a fixed batch time. In this work, it is shown that the optimal operating policy can be split into several time intervals. A...

A controller based on neuro-dynamic programming coupled with a fuzzy ARTMAP neural network for a fed-batch bioreactor was developed to roduce cloned invertase in Saccharomyces cerevisiae yeast in a fed-batch bioreactor. The objective was to find the optimal glucose feed rate profile eeded to achieve the highest fermentation profit in this reactive system where the enzyme expression is repressed...

Abstract The tofu industry produces liquid waste that can cause pollution if it is not processed first. Tofu has the characteristics of containing high organic matter such as BOD and COD so directly discharged into environment, will reduce carrying capacity environment. purpose this study was to determine analyse effect variations in times velocity sequence batch reactor on optimization removal...

Safe and optimal operation of complex production processes is one of the most important research and development problems in process engineering. This problem is the most relevant at the design of the optimal feeding profile of fed-batch chemical reactors due to the nonlinear and unstable dynamical behavior of the processes. This paper shows that how the optimal feeding policy can be determined...

in this article several control schemes for a pilot scale polymerization reactor are proposed and one of them is selected as the most suitable design. the performances of proposed schemes have been compared through computer simulations. the controllers are pid and chosen reaction is polymerization of methyl methacrylate which is highly exothermic.

toxicity of which on biological wastewater processes, humans, and the environment has been proven in various studies. in this study, the half maximal effective concentration (ec 50 ) of growth inhibition of cadmium, lead, and mercury was assessed on dominant bacterial species present in sequencing batch reactors (sbr). methods: biomass sample was obtained from the biological unit of the sbr est...