The effect of direct transesterification methods on the omega-3/6 composition extracts from Phaeodactylum tricornutum was studied. aim this work to identify an extraction method which allowed obtain most suitable profile fatty acids in terms its potential benefits health, particularly if further used food industry. Seven using acids, alkalis, and heterogeneous-catalysts, (namely 1 7, abbreviate...

Selective macroalgae comprising green algae (Ulva lactuca), brown (Padina gymnosphora, Sargassam wightii, Turbinaria ornate) and red (Gracilaria corticata, Halimeda macrolopa, Halymenia dilatata, Gracilaria crassa) were collected from Gulf of Mannar, India. Taxonomic molecular identification affirmed the identity microalgal species. Free fatty acids Fatty Acid Methyl Ester (FAME) synthesis by t...

Fatty acid methyl ester (FAME) and length heterogeneity-polymerase chain reaction (LH-PCR) analyses were used to generate ‘fingerprints’ of FAMEs and eubacterial 16S rDNA sequences characteristic of agricultural soil communities. We hypothesized that pooling data from two methods that characterized different components of soil biological communities would improve the resolution of fingerprints ...

In the relatively pristine ecosystem in Kejimkujik Park, Nova Scotia, methylmercury (MeHg) concentrations in loons, Gavia immer, are among the highest recorded anywhere in the world. This study investigated the influence of bedrock lithology on MeHg concentrations in wetlands. Twenty-five different wetland field sites were sampled over four different bedrock lithologies; Kejimkujik monzogranite...

The authors analyzed the long-chain fatty acids derived from the wax and sterol ester fractions of meibomian secretions from patients with chronic blepharitis and normal individuals. Meibomian secretions were partitioned into separate lipid classes by thin-layer chromatography (TLC). Wax and sterol esters were eluted and transesterified. The liberated fatty acid methyl esters (FAME) were analyz...

Wet washing is a widely used method for biodiesel purification. However, this technique generates large amount of wastewater that needs to be treated afterward, which costly and time-consuming. Thus, solvent-aided crystallization (SAC) with ultrasonic irradiation as solution movement assistance was introduced. This based on the addition 1-butanol enhance purification via crystallization. During...

Abstract The soil microbial community (SMC) and organic matter (SOM) are inherently related sensitive to land‐use changes. Microorganisms regulate essential functions that key SOM dynamics, whereas dynamics define the SMC. To expand our understanding of health, we evaluated biological indicators in long‐term (18‐yr) continuous silage corn ( Zea mays L.), soybean [ Glycine max (L.) Merr.], peren...

The main fatty acids present in lipopolysaccharides from Bacteroides fragilis NCTC 9343 were identified as 13-methyl-tetradecanoic, D-3-hydroxypentadecanoic, D-3-hydroxyhexadecanoic, D-3-hydroxy-15-methyl-hexadecanoic, and D-3-hydroxyheptadecanoic acids. Of these, 13-methyl-tetradecanoic acid is exclusively ester bound, and 3-hydroxy-15-methyl-hexadecanoic acid is exclusively involved in amide ...

The analysis of the fatty acid (FA) profile requires multiple preparation steps, which are lipid extraction followed by derivatization FA into a methyl ester (FAME). procedures time-consuming, and generally require large volumes sample sizes solvents. This report proposes technique for FAME from fresh horse mussels without step extraction. A rapid in situ using N,N-dimethylformamide dimethyl ac...