Ex vivo digestion of proteins and fat in Red Chittagong Cattle milk from Bangladesh was carried out using human gastrointestinal enzymes. This was done to investigate the protein digestion in this bovine breed's milk with an especial focus on the degradation of the allergenic milk proteins; αs1-casein and β-lactoglobulin and also to record the generation of peptides. Lipolysis of the milk fat a...

background: norepinephrine plays a trophic role in the control of cell replication and differentiation in target cells that express adrenergic receptors. methods: in this study, we have tested the influence of infraphysiological, physiological and supraphysiological concentrations (0.0001 nm, 1 nm, 10000 nm) of human norepinephrine on the proliferation of breast cancer cells (human breast adeno...

The action of prostaglandins on cyclic AMP levels in rat epididymal fat pads, isolated fat cells, and other tissues was studied. Fat pads incubated with prostaglandin El (PGE1) contained higher levels of cyclic AMP than controls but lipolysis was not stimulated. However, PGEr did not cause increased cyclic AMP levels in isolated fat cells, suggesting that the stimulatory action occurred in anot...

OBJECTIVE Adipose tissue expressed endogenous cystathionine gamma lyase (CSE)/hydrogen sulfide (H2S) system. H2S precursor inhibited catecholamine stimulated lipolysis. Thus, we hypothesized that CSE/H2S system regulates lipolysis which contributed to the pathogenesis of insulin resistance. METHODS We treated rat adipocyte with DL-propargylglycine (PAG, a CSE inhibitor), L-cysteine (an H2S pr...

The action of prostaglandins on cyclic AMP levels in rat epididymal fat pads, isolated fat cells, and other tissues was studied. Fat pads incubated with prostaglandin El (PGE1) contained higher levels of cyclic AMP than controls but lipolysis was not stimulated. However, PGEr did not cause increased cyclic AMP levels in isolated fat cells, suggesting that the stimulatory action occurred in anot...

Intra-abdominal (IA) fat functionally differs from subcutaneous (SC) adipose tissue, likely contributing to its stronger association with obesity-induced morbidity and to differential response to medications. Drug-induced partial lipodystrophy, like in response to antiretroviral agents, is an extreme manifestation of the different response of different fat depots, with loss of SC but not IA. In...

Hereditary factors may be involved in the pathogenesis of type 2 diabetes. A polymorphism in the hormone-sensitive lipase (HSL) gene (HSLi6) is associated with obesity and diabetes, although it is unknown whether the polymorphism is functional and thereby influences lipolysis. We genotyped 355 apparently healthy nonobese male and female subjects for the HSLi6 polymorphism. Allele 5 was found to...

1. The phospholipid composition of fat-cells prepared from rat epididymal fat-pad was determined. 2. The incorporation of [(32)P]P(i) into the phospholipids of fat-cells incubated in glucose-free medium and the effect of adrenaline and of alpha- and beta-adrenergic blocking agents, were studied. 3. Incorporation of [(32)P]P(i) into fat-cell phospholipid increased with time; incubation with adre...

Body fat distribution is an important predictor of metabolic abnormalities in obese humans. Dysregulation of free fatty acid (FFA) release, especially from upper body subcutaneous adipose tissue, appears to contribute substantially to these metabolic disturbances. Why different individuals preferentially store fat in upper vs. lower body subcutaneous fat or subcutaneous vs. visceral fat is not ...

Since insulin is known to stimulate intracellular hydrogen peroxide production in rat epididymal fat cells, the effects of exogenous hydrogen peroxide on rates of basal and hormone-stimulated lipolysis were investigated in a perifusion system. H2O2 (60 microM) caused a weak and transient stimulation of basal lipolysis that did not interfere with subsequent activation of lipolysis by hormones. M...