Two species of alpha-actinin from rabbit fast skeletal muscles were identified with a monospecific antisera. Designated alpha-actinin1f and alpha-actinin2f, their distribution in muscles does not correlate with histochemically defined fast fiber type. Rather, the presence of each correlates with Z-line width and with the expression of different thin filament Ca2+-regulatory complexes. alpha-Act...

PURPOSE αB-crystallin is a small heat shock protein that acts as a molecular chaperone under various stress conditions. Microtubules, which consist of tubulin, are related to maintain the intracellular organelles and cellular morphology. These two proteins have been shown to be related to the properties of different types of myofibers based on their contractile properties. The response of these...

This study aimed to analyse the expression of myosin heavy chain (MHC) isoforms in bovine muscles, with particular attention to the MHC-2B gene. Diaphragm, longissimus dorsi, masseter, several laryngeal muscles and two extraocular muscles (rectus lateralis and retractor bulbi) were sampled in adult male Bos taurus (age 18-24 months, mass 400-500 kg) and analysed by RT-PCR, gel electrophoresis a...

You know when you’re racing really fast—so fast that your heart is pounding, and you can’t catch breath? Have ever wondered why happens? Imagine are racing—when take a deep breath, breathe in air lungs pull oxygen into blood, then races to hungry leg muscles. All work by muscles make energy creates carbon dioxide waste, which travels back the lungs, we exhale with sigh of relief. As harder run ...

Isozymes of myosin have been localized with respect to individual fibers in differentiating skeletal muscles of the rat and chicken using immunocytochemistry. The myosin light chain pattern has been analyzed in the same muscles by two-dimensional PAGE. In the muscles of both species, the response to antibodies against fast and slow adult myosin is consistent with the speed of contraction of the...

PURPOSE To determine whether the levator palpebrae superioris (LPS) and the retractor bulbi (RB) muscles, which share contractile characteristics with extraocular muscles (EOMs), express fast EOM-specific myosin heavy chain (MyHC) in the rabbit and other mammalian species. METHODS Cryostat sections of rabbit eye and limb muscles were stained by indirect peroxidase immunohistochemical procedur...

Acetylcholinesterase (AChE) expression in fast rat muscles is approximately fourfold higher than in slow muscles. We examined whether different muscle activation patterns are responsible for this difference and whether the calcineurin signaling pathway is involved in AChE regulation. The slow soleus and fast extensor digitorum longus (EDL) muscles were directly or indirectly stimulated by a ton...

A histochemical survey was done on the fiber composition of 12 different locomotory muscles in the lizard Dipsosaurus dorsalis. Three types of fibers were found in all muscles: (1) fast-twitch-glycolytic (FG); (2) fast-twitch-oxidative-glycolytic (FOG); and (3) tonic fibers. Virtually all locomotory muscles contain some tonic fibers. Most muscles have bulk white regions (containing mostly FG fi...