The reviewer appreciate the additional experiment done by the authors about DNA extraction efficiency. However, the efficiency reported in the response seems to be recovery efficiency of DNA associated purification and elution steps, and not efficiency of DNA extraction from fungal cells. In this case, the authors had to spike fungal spores, not naked fungal DNA or PCR amplicons, as an external...

Supercritical CO2 extraction was employed to separate simulated and real bio-oils. Effects of extraction pressure, temperature and adsorbents on distribution coefficient (or enrichment coefficient) of five representative compounds were investigated using a simulated bio-oil, which was composed of acetic acid (AC), propanoic acid (PA), furfural (FR), acetylacetone (AA) and 2-methoxyphenol (MP). ...

The Fermilab crystal-extraction experiment E853 at Tevatron was simulated by Monte Carlo code CATCH [1] tested earlier in the CERN-SPS experiment [2–4]. Predictions for the extraction efficiency, angular scans and extracted beam profiles are presented. Several ideas are proposed and tested by the simulation, how to get in E853 the key information of the extraction experiment: the ”septum width”...

4,4'-Bis(dihexylaminocarbonyl)-2,2'-bipyridine (BDC-Bipy) was synthesized and studied systematically as a chelating reagent for metal ions extraction in supercritical CO(2). The compound showed high extraction efficiency for Co(2+) (100%), Cu(2+) (100%), Cd(2+) (98.2%), and Zn(2+) (100%) ions and good extraction efficiency for Sr(2+) (79.4%) and Pb(2+) (89.8%) when the extraction was performed ...

In this study, extraction of amoxicillin (AMOX) ions from aqueous solution by liquid-liquid extraction (LLE) was investigated. The LLE process by response surface methodology (RSM) was carried out based on central composite design (CCD). The organic phase consisted of extractant and toluene as diluent. In this process, triethylamine (TEA), di-2-ethylhexyl phosphoric acid and mono-2-ethylhexyl p...

The following paper presents a new Content-Based Image Retrieval algorithm. In its realization , we applied the Dual-Tree Complex Wavelet Transform for image features extraction and Hausdorff distance for similarity distance computing between the feature vector of the query-image and the image feature vectors stored in the image database. To estimate its efficiency, we performed some experiment...

water contamination due to the wide variety of pesticides used in agriculture is a global environmental pollution problem. in order to reach at sub-î¼gl-1 levels of detection, an efficient extraction technique is required. a simple, fast and economical method, dispersive liquid-liquid micro extraction (dllme), followed by gas chromatography-mass spectrometry was assessed for determining endosul...

a solid phase extraction sorbent based on polyaniline/graphene nanocomposite is presented. the structure and morphology of synthesized nanocomposite were investigated by fourier transform infrared spectroscopy, field emission scanning electron microscopy, x-ray diffraction and thermal gravimetric analysis. the dispersive solid phase extraction was employed to the isolation and preconcentration ...

Recently, the solid phase extraction technology has received considerable attention considering serious environmental problems and much more contaminants present in the samples at trace level that is different to be analyzed. During the whole process of analyzing sample, the preconcentration of sample is the most complicated and significant step, which typically spend much time, affecting the a...

This work was initiated to study the concentration effects on phenanthrene and pyrene extraction during process of aging in phaeozem and burozem with or without freeze-thawing cycles. 1, 10, and 100 μg g(-1) phenanthrene and pyrene contaminated soils were extracted by 10,000 mg L(-1) surfactant SDBS at various times. The extraction amount decreased with increasing contact time. Aging process co...