An original one dimensional population balance model (PBM)-based of liquid–liquid extraction columns is reported. Compared to existing simulators, ColHySE implements a more realistic description the flow patterns in contactor, and predicts its effect on local droplet–droplet interactions (i.e. breakage coalescence rates). Proper turbulent properties, extracted from single-phase CFD simulations,...

A high performance liquid chromatography method for determining the levels of 4-pregnene-3-ones in tissue containing fat, e.g. in thymus is reported. Following the extraction with chloroform/methanol the fat has been separated from the steroidal-fraction by using disposable extraction-columns. The steroidal-fraction has been analysed in two separation systems, on a RP18-column with a methanol/w...

Extraction of DNA from Staphylococcus aureus is often difficult and less efficient. In this study, the relative recovery of extraction of staphylococcal DNA from pure culture was compared using rapid lysis, bashing beads and commercial silica column methods. For both methicillin resistant and sensitive Staphylococcus aureus the DNA extracted by rapid lysis method was found to have higher value ...

The interconversions of C1-substituted folates form the core of plant one carbon metabolism. An analytical method for extraction, purification and liquid chromatography−electrospray-ionization mass spectrometry detection of low level, labile tetrahydrofolate monoglutamates from Arabidopsis thaliana was developed in this study. Under gold fluorescent light, folates were extracted by homogenizing...