background and objectives: during past decades hansenula polymorpha has attracted global attention for the expression of recombinant proteins due to its high growth rate, minimal nutritional porequirements and use of methanol as a low cost inducer. materials and methods: the corresponding nucleotide sequences for the expression of heterologous genes in hansenula poylmorpha were extracted and as...

introduction: g-proteins have an important role in the cell signaling of numerous receptors. the situation of g-proteins in health and disease and their critical role in the development of diabetic side effects is an interested scientific field. here, the changes in the expression of g-protein subunits (gαi, gαs and gβ) were evaluated in hyperglycemic situation of pc12 cells as a cellular model...

background: to evaluate vdr gene variation and its interaction with immune regulatory molecules in osteoporosis. methods: totally 205 pre and postmenopausal women were recruited in the study. after an overnight fast, peripheral blood was taken and centrifuged to sprat serum for measurement of serum parathyroid hormone, 25 hydroxyvitamin d, osteocal­cin and cross laps. the fok i polymorphism in ...

gene expression profiling of ovarian carcinoma tissues has shown an increase of four-fold expression of sort1 gene. sortilin 1 (ntr-3) is a 95-100 kda protein normally expressed in heart, brain, placenta, skeletal muscle, spinal cord, thyroid, and testis. however, its expression has never been reported in normal ovary. here, we report expression of sortilin 1 in ovarian carcinoma tissues both a...

asthma is a chronic inflammatory disorder of the airways. the stress is a factor for asthma which indicates a disorder in the function of communicational mediators of nervous and  immunological systems  such  as  neurotransmitters.  a  study  indicated  that  blood serotonin concentration increases in asthmatic patients. other study indicates that one kind of the serotonin  receptors, named 5ht...

abstract        background and objective: various cellular factors affect the process of hiv activity. one of these cellular factors are structures known as microrn that are expected to be involved in controlling hiv replication and infectivity. the expression of one or a set of them may represent the patient's clinical conditions. in this study,  the expression of mir-29a and mir-29b invo...

an important step in considering of gene expression data is obtained groups of genes that have similarity patterns. biclustering methods was recently introduced for discovering subsets of genes that have coherent values across a subset of conditions. the las algorithm relies on a heuristic randomized search to find biclusters. in this paper, we introduce biclustering las algorithm and then appl...

the µ (mu) opioid receptors, which mediate the effects of morphine, are widely distributed in brain. the purpose of this study was to design a simple expression system for rat µ-receptor in escherichia coli (bl21). in this laboratory study, rat µ-receptor cdna was isolated from pcdna3 vector using xba1 and hind3 restriction enzymes. pet-15b was digested by nco1 restriction enzyme. µ-receptor cd...

trehalose (a-d-glucosyl-[1,1]-a-d-glucopyranoside) is involved in mechanisms that coordinate metabolism with plant growth adaptation and development. the main objective of the current work was to find out whether trehalose feeding affects the expression of two genes involved in photosynthesis: one gene coding for photosystem1 subunit h (ps1-h) and the other for the light harvesting complex b1 (...