نتایج جستجو برای: ex vivo expansion

تعداد نتایج: 498294  

2015
Nazmul Haque Noor Hayaty Abu Kasim Mohammad Tariqur Rahman

Mesenchymal stem cells (MSCs) are considered a potential tool for cell based regenerative therapy due to their immunomodulatory property, differentiation potentials, trophic activity as well as large donor pool. Poor engraftment and short term survival of transplanted MSCs are recognized as major limitations which were linked to early cellular ageing, loss of chemokine markers during ex vivo ex...

Journal: :American journal of physiology. Heart and circulatory physiology 2004
Tao-Sheng Li Masanori Hayashi Ze-Lin Liu Hiroshi Ito Akihito Mikamo Akira Furutani Masunori Matsuzaki Kimikazu Hamano

Ex vivo expansion of stem cells might be a feasible method of resolving the problem of limited cell supply in cell-based therapy. The implantation of expanded CD34(+) endothelial progenitor cells has the capacity to induce angiogenesis. In this study, we tried to induce angiogenesis by implanting expanded CD117(+) stem cells derived from mouse bone marrow. After 2 wk of culture with the additio...

Journal: :iranian journal of pediatric hematology and oncology 0
f skandari department of hematology and blood banking, faculty of medical science, tarbiat modares university, tehran, iran a allahverdi department of hematology and blood banking, faculty of medical science, tarbiat modares university, tehran, iranسازمان اصلی تایید شده: دانشگاه تربیت مدرس (tarbiat modares university) h nasiri hematology-oncology and stem cell transplantation research center, tehran university of medical science, tehran, iranسازمان اصلی تایید شده: دانشگاه تربیت مدرس (tarbiat modares university) m azad department of medical laboratory sciences, faculty of allied medicine, qazvin university of medical science, qazvin, iraسازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (tehran university of medical sciences) n kalantari department of hematology and blood banking, faculty of medical science, tarbiat modares university, tehran, iranسازمان اصلی تایید شده: دانشگاه علوم پزشکی قزوین (qazvin university of medical sciences) m soleimani department of hematology and blood banking, faculty of medical science, tarbiat modares university, tehran, iranسازمان اصلی تایید شده: دانشگاه تربیت مدرس (tarbiat modares university)

background the aim of this study was the ex vivo expansion of umbilical cord blood hematopoietic stem cells on biocompatible nanofiber scaffolds. materials and methods cd133+ hematopoietic stem cells were separated from umbilical cord blood using midimacs (positive selection) system by means of monocolonal antibody cd133 (microbeads) subsequently, flowcytometry method was done to assess the pur...

Journal: :The Journal of clinical investigation 2014
Hal E Broxmeyer

In this issue of the JCI, Chaurasia and colleagues report an impressive ex vivo expansion of HSCs from human cord blood (CB) using cytokines and altering epigenetic modifications. The application of this protocol provides information that has potential for clinical consideration. The enhanced expansion of CB HSCs is a substantial advance over recent work from the Chaurasia and Hoffman group, in...

2011
Marylyn M. Addo Marcus Altfeld Diana M. Brainard Almas Rathod Alicja Piechocka-Trocha Ulgen Fideli Joseph Mulenga Erin Shutes Donna Marie L. Alvino Eric Hunter Susan A. Allen Bruce D. Walker

Human immunodeficiency virus type 1 (HIV-1)-specific T cell responses were characterized in a blinded study involving infected individuals and their seronegative exposed uninfected (EU) partners from Lusaka, Zambia. HIV-1-specific T cell responses were detected ex vivo in all infected individuals and amplified, on average, 27-fold following in vitro expansion. In contrast, no HIV-1-specific T c...

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