Regeneration of in vitro multiple shoots was achieved through organogenesis on MS supplemented with 2.0 mg/l BAP and 0.5 Kn from cotyledonary leaf explants two local varieties eggplant (Solanum melongena L.). Elongation regenerated obtained growth regulator free MS. In root induction excised less effective or without plant regulators. On the other hand treated 10 mM IBA for 5 min were found to ...

an efficient and cost effective protocol for in vitro propagation of pineapple (ananas comosus var. queen) has been developed. in the proliferation stage, agar based murashige and skoog (ms) media was supplemented with 3.0 mg/l benzyleaminopurine (bap), 0.5 mg/l indole acetic acid (iaa) and 50 mg/l adenine sulphate as rbc design experiment. two approaches were taken to reduce the chemical cost ...

This study surveyed a rapid, efficient and reproducible protocol for in vitro shoot regeneration and rooting by different explants and different concentration of BAP. Due to optimization of shoot regeneration, two media including of MS and modified MS (MS salts with double concentration of CaC21, and B5 vitamins), different explants such as decapitated embryo axes, epicotyls and cotyledonary no...

ABSTRACT: Micropropagation is one technology to propagate endemic species of the Ferruginous Rupestrian Grasslands when in vitro genetic conservation sought. The present study aimed assess breaking dormancy, establishment, multiplication, elongation, rooting, and acclimatization Mimosa calodendron from culture a temporary immersion bioreactor system. seeds used for experiments were plants origi...

Elms are threatened by Dutch elm disease, and conservation methods needed to protect their genetic diversity. Cryopreservation of dormant buds allows large numbers genotypes be conserved with small space requirements minimal upkeep. through slow controlled cooling was tested for both species native Finland, Ulmus glabra laevis. Regeneration the thawed micropropagation studied on different basal...

EFFECTS OF CARBON DIOXIDE ENRICHMENT AND IRRADIANCE ON EX VITRO ROOTING, ACCLIMATIZATION AND SUBSEQUENT GROWTH OF MICROPROPAGATED APPLE AND BLUEBERRY. Marvin Pritts and Dorcas Isuta*, Cornell University, Fruit and Vegetable Science Department. 134A Plant Science Building, Ithaca, NY 14850 Previous findings reveal that rooting and acclimatization of apple and blueberry plants is often difficult ...