This paper presents an innovative portable chip-based RT-PCR system for amplification of specific nucleic acid and detection of RNA-based viruses. The miniature RT-PCR chip is fabricated using MEMS (Micro-electro-mechanical-system) techniques, and comprises a micro temperature control module and a PDMS (polydimethylsiloxane)-based microfluidic control module. The heating and sensing elements of...

Characteristic Strain GenBank accession no. KT818796 KT818795 KT818793 KT818794 Country of isolation Niger Guinea Senegal Mauritania Region Zinder Labé Thies Assaba Sex M M F F Age at diagnosis, y 1.3 1.7 3 1.6 Paralysis onset date 2013/02/26 2013/04/24 2014/03/17 2014/05/06 Date of first sampling 2013/03/06 2013/04/29 2014/03/26 2014/05/22 Fever at onset of paralysis yes yes yes yes Progressiv...

With the increase in number of electric vehicles (EVs) and developments their related charging infrastructures, consumers have still some concerns about limiting factors EV industry such as battery life, station availability, grid capacity, limited driving range, slow batteries. Although solutions are proposed for these limitations, they not sufficiently efficient cost-effective. Moreover, EVs ...

The full-length genome sequence of a human enterovirus 71 (EV71) strain (EV71/CZTN01/CHN/2017) was isolated from a throat swab from a child in Changzhou, China, in 2017. According to the phylogenetic analyses, the full-genome sequence in this study belongs to sub-subgenotype C4a.

Picornaviruses (PV) include human rhinovirus (HRV), the primary cause of the common cold, and the enteroviruses (EV), which cause serious diseases such as poliomyelitis, meningoencephalitis, and systemic neonatal disease. Although no compounds for PV infections have been approved in the United States, pirodavir was one of the most promising capsid-binding compounds to show efficacy in human cli...

Enterovirus (EV) 71 has emerged as a primary cause of severe neurologic enterovirus infection in the aftermath of the global polio eradication effort. Eleven subgenotypes of EV71 exist, the C4 subgenotype being associated with large outbreaks in Asia with high mortality rates. This subgenotype has rarely been reported in Europe. In the period between 1 January 2009 and 31 December 2013 a total ...

Los enterovirus constituyen un amplio grupo de virus de distribución ambiental. Afectan preferentemente a la población infantil con enfermedades respiratorias, gastrointestinales, cuadros exantemáticos, febriles y neurológicos. Se trasmiten fácilmente de persona a persona por la vía fecal-oral y presentan un período de incubación de 1-3 días1. Dentro de los más de 100 enterovirus conocidos, el ...