Alkaline phosphatase-conjugated (AP) 26-base oligonucleotide DNA probes were compared with the same probes labeled with y_32p for the identification of heat-labile (LT) and heat-stable (ST) enterotoxigenic Escherichia coli (ETEC). The AP oligonucleotide probes were as sensitive as the radiolabeled (RL) probes in detecting LT and STA-2 target cell DNA, but the AP ST probe, which differed from ST...

Probiotic bacteria may provide protection against intestinal damage induced by pathogens, but the underlying mechanisms are still largely unknown. We investigated whether Bifidobacterium animalis MB5 and Lactobacillus rhamnosus GG (LGG) protected intestinal Caco-2 cells from the inflammation-associated response induced by enterotoxigenic Escherichia coli (ETEC) K88, by inhibiting pathogen attac...

Enterotoxigenic Escherichia coli (ETEC) is a prevalent cause of traveler's diarrhea and infant mortality in third-world countries. Heat-labile enterotoxin (LT) is secreted from ETEC via vesicles composed of outer membrane and periplasm. We investigated the role of ETEC vesicles in pathogenesis by analyzing vesicle association and entry into eukaryotic cells. Fluorescently labeled vesicles from ...

Volume 3, no. 3, e00564-15, 2015. Page 1: The title should read as given above and the number of enterotoxigenic Escherichia coli (ETEC) serogroup O6 strains in the study should be given as nine throughout because one of the ETEC strains was confirmed to be a non-O6 strain. Page 1: The third and fourth sentences of the second paragraph should read as follows. “The average size of the ETEC genom...

Enterotoxigenic Escherichia coli (ETEC) is a leading cause of death due to diarrheal illness among young children in developing countries, and there is currently no effective vaccine. Many elements of ETEC pathogenesis are still poorly defined. Here we demonstrate that YghJ, a secreted ETEC antigen identified in immunoproteomic studies using convalescent patient sera, is required for efficient ...

Direct detection of enterotoxigenic Escherichia coli (ETEC) in mixed cultures by DNA filter assay was affected by the presence of other bacteria in the cultures. By shortening incubation to 6 h, bacterial overgrowth was minimized, allowing direct detection of ETEC in stools or mixed cultures initially containing 0.2 to 3% ETEC.

Enterotoxigenic Escherichia coli (ETEC) is one of the major causes of infectious diarrhea in developing countries. In order to characterize the molecular features of human ETEC isolates from Korea, we investigated the profiles of enterotoxin and colonization factor (CF) genes by polymerase chain reaction (PCR) and performed multilocus sequence typing (MLST) with a total of 291 ETEC strains. The...

Preventive vaccines against enterotoxigenic Escherichia coli (ETEC) are being developed, many of which target common fimbrial colonization factors as the major constituent, based on empirical evidence that these function as protective antigens. Particularly, passive oral administration of ETEC anti-fimbrial antibodies prevent ETEC diarrhea. Little is, however, known regarding the specific mecha...

Porcine Post-Weaning Diarrhea (PWD) continues to be one of the most important swine diseases worldwide. Entero toxigenic Escherichia coli (ETEC) strains are the primary cause of PWD. The key virulence factors of ETEC in PWD are bacterial fimbrial adhesins and enterotoxins. Adhesions mediate ETEC bacteria initial attachment to pig epithelial cells and subsequent colonization at pig small intesti...

A cohort of 321 children was followed from birth up to 2 years of age to determine the incidence of enterotoxigenic Escherichia coli (ETEC) in Bangladesh. The average number of diarrheal days and incidence rates were 6.6 and 2.3/child/year, respectively. ETEC was the most common pathogen and was isolated in 19.5% cases, with an incidence of 0.5 episode/child/year. The prevalence of rotavirus di...