نتایج جستجو برای: est ssr markers

تعداد نتایج: 251106  

2017
Yue Zhang Xue Zhang Yue-Hua Wang Shi-Kang Shen

Transcriptome sequences generated by next-generation sequencing (NGS) technologies can be utilized to rapidly detect and characterize a large number of gene-based microsatellites from different plants. Rhododendron rex Lévl. is a perennial woody species from the family Ericaceae and an endangered plant with high ornamental value endemic to Southwestern China. Nevertheless, the genetic and genom...

2014
V. GOURI SHANKAR P. VENKATA RAMANA RAO P. BINDU PRIYA M. V. NAGESH KUMAR A. V. RAMANJANEYULU VISHNUVARDHAN REDDY Akshaya K. Biswal SABRAO J. Breed

Maintenance of genetic purity of a hybrid is essential to exploit its full potential. Genetic purity is generally assessed by conducting grow out test (GOT) where morphological and floral characters are analyzed, however results may be influenced by the environment. Further, it is expensive and time consuming. DNA based markers are rapid, reliable and cost effective for hybrid purity assessment...

2010
Raj Kumar Joshi Ananya Kuanar Sujata Mohanty Enketeswara Subudhi Sanghamitra Nayak

Turmeric (Curcuma longa L.) (Family: Zingiberaceae) is a perennial rhizomatous herbaceous plant often used as a spice since time immemorial. Turmeric plants are also widely known for its medicinal applications. Recently EST-derived SSRs (Simple sequence repeats) are a free by-product of the currently expanding EST (Expressed Sequence Tag) databases. SSRs have been widely applied as molecular ma...

Journal: :Molecules 2014
Jin Zhang Wenge Ma Xiaomin Song Qiaohong Lin Jian-Fang Gui Jie Mei

Yellow catfish (Pelteobagrus fulvidraco) is one of the most important freshwater fish due to its delicious flesh and high nutritional value. However, lack of sufficient simple sequence repeat (SSR) markers has hampered the progress of genetic selection breeding and molecular research for yellow catfish. To this end, we aimed to develop and characterize polymorphic expressed sequence tag (EST)-S...

2013
Touming Liu Siyuan Zhu Lili Fu Qingming Tang Yongting Yu Ping Chen Mingbao Luan Changbiao Wang Shouwei Tang

Ramie (Boehmeria nivea L. Gaud) is one of the most important natural fiber crops, and improvement of fiber yield and quality is the main goal in efforts to breed superior cultivars. However, efforts aimed at enhancing the understanding of ramie genetics and developing more effective breeding strategies have been hampered by the shortage of simple sequence repeat (SSR) markers. In our previous s...

Journal: :Genetics and molecular research : GMR 2014
J J Lu J Y Kang S R Ye H Z Wang

Expressed sequence tag (EST)-derived simple sequence repeat (SSR) markers could enrich the current resource of molecular markers. In this study, microsatellite markers were developed for Dendrobium nobile Lindl. by mining the ESTs. Twenty-eight EST-SSRs amplified 2 to 6 nucleotide repeats with a mean number of 2.82 alleles per locus. The observed and expected heterozygosities per locus ranged f...

Journal: :In silico biology 2006
B. Jayashree Ramu Punna P. Prasad Kassahun Bantte C. Tom Hash Subhash Chandra David A. Hoisington Rajeev K. Varshney

Simple sequence repeats (SSRs) or microsatellites are an important class of molecular markers for genome analysis and plant breeding applications. In this paper, the SSR distributions within ESTs from the legumes soybean (Glycine max, representing 135.86 Mb), medicago (Medicago truncatula, 121.1 Mb) and lotus (Lotus japonicus, 45.4 Mb) have been studied relative to the distributions in cereals ...

2012
Mathilde Allegre Xavier Argout Michel Boccara Olivier Fouet Yolande Roguet Aurélie Bérard Jean Marc Thévenin Aurélie Chauveau Ronan Rivallan Didier Clement Brigitte Courtois Karina Gramacho Anne Boland-Augé Mathias Tahi Pathmanathan Umaharan Dominique Brunel Claire Lanaud

Theobroma cacao is an economically important tree of several tropical countries. Its genetic improvement is essential to provide protection against major diseases and improve chocolate quality. We discovered and mapped new expressed sequence tag-single nucleotide polymorphism (EST-SNP) and simple sequence repeat (SSR) markers and constructed a high-density genetic map. By screening 149 650 ESTs...

2014
Mian Zhang Weihua Mao Guoping Zhang Feibo Wu

Tibetan annual wild barley is rich in genetic variation. This study was aimed at the exploitation of new SSRs for the genetic diversity and phylogenetic analysis of wild barley by data mining. We developed 49 novel EST-SSRs and confirmed 20 genomic SSRs for 80 Tibetan annual wild barley and 16 cultivated barley accessions. A total of 213 alleles were generated from 69 loci with an average of 3....

2016
Xin Yin Yunqiang Yang Yongping Yang

PREMISE OF THE STUDY Expressed sequence tag-simple sequence repeat (EST-SSR) markers were developed using Illumina sequencing for further genetic diversity studies of Stipa purpurea (Poaceae). METHODS AND RESULTS Twenty-nine polymorphic and eight monomorphic EST-SSR loci were developed and characterized in 90 individuals from nine S. purpurea populations. The number of alleles per locus range...

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