نتایج جستجو برای: escherichiacoli
تعداد نتایج: 89 فیلتر نتایج به سال:
A mechanistic model for lactose/H(+) symport via the lactose permease of Escherichia coli proposed recently indicates that the permease must be protonated to bind ligand with high affinity. Moreover, in the ground state, the symported H(+) is shared between His-322 (helix X) and Glu-269 (helix VIII), whereas Glu-325 (helix X) is charge-paired with Arg-302 (helix IX). Substrate binding at the ou...
Extra-intestinal pathogenic E. coli cause urinary tract infections (UTIs), newborn meningitis, abdominal sepsis and septicemia. UTIs affect millions of women annually, and result in significant health care costs and morbidity worldwide. Uropathogenic E. coli (UPEC) is the predominant urinary tract pathogen, causing up to 85% of UTIs. Despite appropriate therapy, recurrent episode of UTI are com...
فلاونوئیدها نقش مهمی را به عنوان سیگنال‌های مولکولی در تشکیل گرهها توسط ریزوبیومهای همزیست بازی میکنند. لوتئولین یکی از مهمترین فلاونوئیدها است که توسط بذرهای در حال جوانهزنی یونجه ترشح میشود. همچنین به نظر میرسد سطح شوری بالا بدون حضور فلاونوئید موجب افزایش بیان ژنهای گرهزایی میشود. بنابراین، در این آزمایش تأثیر لوتئولین، ترشحات بذر و شوری بر بیان ژن گرهزایی دو سویه حساس و مقاوم به ش...
Abstract Background: By increasing resistance of pathogenic bacteria to antibiotics, many attempts have been made by the researchers to find new compounds as a substitute for non-effective antibiotics. The aim of this research is a comparative study of antibacterial properties of four species on standard strains of Escherichiacoli, Shigellaflexneri, Klebsiella pneumonia, Candida and Candida alb...
Studies on Staphylococcusaureus and Staphylococcusintermedius from dog and cat, and also on Staphylococcusaureus from wound and pyoderma infections, have shown a correlation between the site of microbial infection and antimicrobial susceptibility. Both the methanolic extract concentrate of Garcinia kola (Heckel) seeds and natural honey have been associated with activity on bacterial isolates fr...
Production of correctly folded and biologically active proteins in Escherichiacoli can be a challenging process. Frequently, proteins are recovered as insoluble inclusion bodies and need to be denatured and refolded into the correct structure. To address this, a refolding screening process based on a 96-well assay format supported by design of experiments (DOE) was developed for identification ...
BACKGROUND Inflammation-induced pain amplification and hypersensitivity play a role in the pathophysiology of numerous clinical conditions. Experimental endotoxemia has recently been implemented as model to analyze immune-mediated processes in human pain. In this study, we aimed to analyze dose- and time-dependent effects of lipopolysaccharide (LPS) on clinically-relevant pain models for muscul...
BACKGROUND Long-read sequencing is rapidly evolving and reshaping the suite of opportunities for genomic analysis. For the MinION in particular, as both the platform and chemistry develop, the user community requires reference data to set performance expectations and maximally exploit third-generation sequencing. We performed an analysis of MinION data derived from whole genome sequencing of Es...
شیر و محصولات لبنی، مهم ترین محصول غذایی با منشاء حیوانی هستند و اغلب به عنوان یک محیط کامل توصیف می¬شوند، به این دلیل که شامل پروتئین، قند، چربی و مواد معدنی هستند. شیر که یک جزء اصلی در رژیم غذایی انسان محسوب می¬شود، به عنوان یک محیط خوب برای رشد بسیاری از میکروارگانیسم¬ها مخصوصا باکتری¬های پاتوژن عمل می¬کند. شیر خام سالم در صورتی¬که در شرایط بهداشتی دوشیده شود به طور نسبی میکروارگانیسم¬های ک...
The impact of discharged treated brewery effluent stream on the microbial andphysicochemical qualities Ikpoba River which is serving as a receptacle for treatedwastewater was investigated. Serial dilution, pour plate and multiple tube technique wereemployed in evaluating culturable diversity water samples thetreated wastewater. physicochemical attributes freshwater effluentsamples were ascertai...
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