Escherichia coli strain K-12 substrain HMS174 is an engineered descendant of the E. coli K-12 wild-type strain. Like its ancestor, it is an important organism in biotechnological research and is used in fermentation processes for heterologous protein production. Here, we report the complete genome sequence of E. coli HMS174 (ATCC 47011).

background and objective: to evaluate extended spectrum betalactamase (esbl) positive strains of klebsiella pneumonia and escherichia coli in positive bacterial cultures. materials and methods: in this analytical cross-sectional study, between march 2006 and march 2007, 170 bacterial isolates including 133 cases of e. coli and 37cases of k. pneumonia were examined. all cases underwent double di...

conclusions ctx-m was the most prevalent esbl genotype in uropathogenic e. coli (upec) isolated from uti. in addition, a high frequency of qnr genes among esbl-producing e. coli was identified in this study. in order to avoid treatment failures, we recommend using phenotypic and molecular methods to diagnose these enzymes and qnr genes. results the highest sensitivity was seen to imipenem (96.7...

conclusions the impact of sugars and other factors on biofilm formation can vary depending on the type of bacteria present. results results showed that the presence of sugars has no effect on the bacterial growth rate; all three concentrations of sugars were hydrophilic and demonstrated a high affinity toward binding to the surfaces. background bacteria utilize various methods in order to live ...

conclusions our present study shows that sugar beet molasses is a source of plp and l-ser; and hence, suggests the general application of the approach to produce l-trp more economically. results consequently, the potassium phosphate buffer system was considered as the suitably buffered reaction medium. the concentration of l-trp produced in reaction medium was 0.3 mm and 0.12 mm for glucose and...

conclusions reteplase was expressed in e. coli top 10 after activation of pbad/giiia promoter region by arabinose and optimized. results the obtained recombinant plasmid was sequenced to confirm the presence and correct framing of reteplase gene regarding the expression of reteplase. maximum production of this enzyme was obtained under the following condition: 0.0002% l-arabinose at 37°c for 2 ...

background development of antibiotic resistance among pathogenic bacteria motivates the researchers to search for newer antimicrobial agents. objectives in the present study, antibacterial effects of cuminum cyminum linn. essential oil against multidrug resistant (mdr) escherichia coli strains isolated from urinary tract infections were studied, using microdilution method. materials and methods...

Escherichia coli strain K-12 substrain RV308 is an engineered descendant of the K-12 wild-type strain. Like its ancestor, it is an important organism in biotechnological research and is heavily used for the expression of single-chain variable fragments. Here, we report the complete genome sequence of E. coli K-12 RV308 (ATCC 31608).

background extended-spectrum beta-lactamases (esbls) are a group of enzymes that hydrolyze antibiotics, including those containing new cephalosporins, and they are found in a significant percentage of escherichia coli and klebsiella pneumoniae strains. with the widespread use of antibiotics, difficulties with infection therapy caused by drug resistant organisms, especially those that have acqui...

SYSTEM of genetic transduction has been discovered in the sexually fertile A K-12 strain of Escherichia coli. This transduction is mediated by lambda, a temperate phage for which K-12 is normally lysogenic. The distinctive features of the lambda-K-12 system include the following: (1) The transductions are limited to a cluster of genes for galactose fermentation. The GaZ loci are closely linked ...