background : the emergence and increase in the incidence of extended-spectrum beta lactamase (esbl) producing escherichia coli has become an emerging challenge especially in hospitalized patients with uti. the aim of the present study was to survey the frequency of bla ctx-m genotype in esbl producing e. coli isolated from hospitalized patients with uti and determination of their antibiotic res...

background: the criteria for the quality control of iranian fresh produce are the total microbial count, enterococcal count and the detection of escherichia coli. the aim of this study was to assess microbial contamination of fresh vegetable and salad samples consumed in tehran, iran. methods: from may to october 2013, freshly vegetable (n=100) and salads (n=100) samples were randomly collected...

conclusions this is the first report of expression of cmv cp gene in iran, which is important for the preparation of anti-cmv antibody and paving the way for the use of the virus coat protein as a nanomaterial. results the identity of the expressed protein was confirmed by immunoassays such as western blot, dot-immunobinding assay (diba) and enzyme-linked immunosorbent assay (elisa) by the use ...

background and purpose: brain-derived neurotrophic factor (bdnf) is present in the hippocampus and cerebral cortex in high concentrations and has an important role in development, maintenance and regeneration of the nervous system. the aim of this study was to produce active bdnf in e.coli using transforming plasmid. methods: to construct an expression plasmid of bdnf, a gene coding for it was ...

the present study was conducted to investigate antimicrobial resistant pattern of escherichia coli (e. coli) strains isolated from clinical specimens of jordanian pediatric patients during the period from january to december 2008. a total of 444 e. coli strains were isolated from clinical specimens and tested for their susceptibility to different antimicrobial drugs. overall, high resistance ra...

background and objectives: bacteria need iron for growth and most of them can actively acquire fe ions using especial iron-chelating proteins which named siderophores. we aimed to determine the frequencies of iuca, iron and irp2 genes in the uropathogenic escherichia coli (upec) isolates. we also analyzed the effects of siderophore genes beside iron supplements on growth rate of the isolates. m...

background: the emergence and propagation of different phylogenetic groups of antimicrobial-resistant e. coli have become a worldwide health concern in human and veterinary medicine. therefore, the evaluation of the phylogenetic distribution of antibiotic-resistant e. coli is important for therapeutic and economic purposes. the aims of this study were to determine phylogenetic groups and patter...

background and objectives: the therapeutic options for diseases caused by escherichia coli are limited. in this study we investigated the presence of virulence factors among enteropathogenic escherichia coli (epec) strains and their antibiotic resistance patterns. the isolates were also checked for the presence of class1 integrons and gene cassettes. materials and methods: this study included 7...

conclusions the analysis recommended that ta genes are prevalent in clinical isolates of e. coli strains. the analysis revealed that hipba ta system is associated with biofilm formation. results the results of the analysis revealed that 107 e. coli isolates were potent for biofilm formation by cra. the findings by microtiter plates showed that 102 e. coli isolates were biofilm producers. the re...