Colibacillosis caused by E.coli is one of the most common illnesses in commercial chickens and induce high financial losses in the poultry industry due to morbidity, mortality and antibiotic expenses because of overuses. E.coli can mostly cause disease as a secondary or opportunistic pathogen, while certain strains with specific virulence factors even as primary pathogen can cause colibacillosi...

BACKGROUND In a biological system, an engineered nanomaterial (ENM) surface is altered by adsorbed proteins that modify ENM fate and toxicity. Thus far, protein corona characterizations have focused on protein adsorption, interaction strength, and downstream impacts on cell interactions. Given previous reports of Ag ENM disruption of Cu trafficking, this study focuses on Ag ENM interactions wit...

Hsc66 and Hsc20 comprise a specialized chaperone system important for the assembly of iron-sulfur clusters in Escherchia coli. Only a single substrate, the Fe/S template protein IscU, has been identified for the Hsc66/Hsc20 system, but the mechanism by which Hsc66 selectively binds IscU is unknown. We have investigated Hsc66 substrate specificity using phage display and a peptide array of IscU....

In vitro antibacterial activity of hydro-alcoholic extract of four selected medicinal plants was screened using agar well diffusion and broth dilution techniques. The four medicinal plants were leaf of Buddleja polystachya and Cordia Africana, stem bark of Albizia gummifera and areal part of Thymus schimperi. Antibacterial activity was assessed against: Pasteurella gallinarum, Salmonella gallin...

Introduction and objective: Otitis externa, commonly called ear disease, is characterized by inflammation of the external ear canal. The aim of this study was to evaluate the antimicrobial potential of Cassia fistula flowers, leaves and bark extracts against Staphylococcus aureus, Proteus mirabilis, Escherchia coli, Pseudomonas aeruginosa, Acinetobacter sp., and Candida albicans, pathogens caus...

A total of three carbazole alkaloids and essential oil from the leaves of Murraya koenigii (Rutaceae) were obtained and examined for their effects on the growth of five antibiotic resistant pathogenic bacteria and three tumor cell lines (MCF-7, P 388 and Hela). The structures of these carbazoles were elucidated based on spectroscopy data and compared with literature data, hence, were identified...

We have engineered a soluble, stable two-chain dimeric streptavidin (TCD) in Escherchia coli. Examination of the three-dimensional structure of streptavidin aided by empirical binding free-energy calculations helped us to select mutations at subunit interfaces that dissociate the native tetramer and stabilize the desired dimer. We chose positions W120, L124, V125 and H127 and mutated them to 12...

UNLABELLED Ear infection is one of the common diseases occurring throughout the world. Different etiological agents are responsible for ear infections. AIM To assess the antimicrobial potential of Terminalia arjuna leaves and bark extracts against Staphylococcus aureus, Acinetobacter sp., Proteus mirabilis, Escherchia coli, Pseudomonas aeruginosa and Candida albicans, pathogens causing ear in...

Escherchia fergusonii and Enterobacter taylorae have recently been recognised as emerging pathogens. Formerly known as Enteric Group 10 and 19, these are new species in the family Enterobacteriaceae. By DNA hybridization, strains of E.fergusonii were found to be 90-97% related to the type strain (holotype) ATCC 35469 and most closely related to E. coli. E. fergusonii can be differentiated from ...

Sanitation of hatching eggs is an important area of research due to the need for an effective, economical, and safe method of egg sanitation. Improved hatching egg sanitation is an important part of an overall pathogen reduction program within integrated poultry operations. This must be accomplished without disturbing the cuticle of the egg, which can decrease hatchability. The ability of ultra...