Streptomycin- and sulfonamide-resistant Erwinia amylovora CA3R from California contained an 8.7-kb plasmid, pEa8.7, with a sulII-strA-strB resistance region; furthermore, PCR, sequencing, hybridization, and restriction analyses showed that pEa8.7 was closely related or identical to broad-host-range plasmid RSF1010. Although RSF1010 has been found in a variety of bacteria, this is the first repo...

Four methods were tested to assess the fire-blight disease response on grafted pear plants. The leaves of the plants were inoculated with Erwinia amylovora suspensions by pricking with clamps, cutting with scissors, local infiltration, and painting a bacterial suspension onto the leaves with a paintbrush. The effects of the inoculation methods were studied in dose-time-response experiments carr...

All strains of Erwinia amylovora characterized carry a medium-size plasmid of 29 kilobases (pEA29). We mapped this plasmid with various restriction enzymes, cloned the whole DNA into an Escherichia coli plasmid, and subcloned restriction fragments. These DNA species were used for identification of E. amylovora after handling of strains in the laboratory and also in field isolates. About 70 stra...

Quorum sensing via an N-acyl homoserine lactone (HSL) pheromone controls the biosynthesis of a carbapenem antibiotic in Erwinia carotovora. Transcription of the carbapenem biosynthetic genes is dependent on the LuxR-type activator protein, CarR. Equilibrium binding of a range of HSL molecules, which are thought to activate CarR to bind to its DNA target sequence, was examined using fluorescence...

The lysis of Erwinia carotovora by bacteriophage has been reported by Coons and Kotila (1925), but the morphological characteristics of the virus could not be studied at that time, prior to the methods of electron microscopy, and some of the important physiological characteristics have not been investigated. In undertaking to obtain further data on these aspects of the problem, we have obtained...

Serological assays were used to estimate the proportion of Acalymma vittata (F.) that harbored Erwinia tracheiphila (E. F. Smith) Holland, the causal agent of bacterial wilt in cucurbits. Theseproportionswere related to theproportionofA. vittata that transmitteddisease in singlebeetle caged bioassays. The serological assays classiÞed beetles as harboring the bacteria when the titer was above '1...

Erwinia mallotivora was isolated from papaya infected with dieback disease showing the typical symptoms of greasy, water-soaked lesions and spots on leaves. Phylogenetic analysis of 16S rRNA gene sequences showed that the strain belonged to the genus Erwinia and was united in a monophyletic group with E. mallotivora DSM 4565 (AJ233414). Earlier studies had indicated that the causal agent for th...

Increased yields of L-asparaginase from Erwinia aroideae NRRL B-138 were obtained by medium enrichment techniques coupled with aeration optimization.