We report on the application of site-directed spin labeling (SDSL) and electron paramagnetic resonance (EPR) spectroscopy to study possible oligomerization of the bacterial toxin colicin A (ColA) upon membrane insertion in vitro and in vivo. We applied SDSL-EPR protocols and optimized experimental conditions to perform continuous wave EPR experiments and double electron-electron resonance dista...

We report the first absorption-based single-chip transceiver for electron paramagnetic resonance (EPR) spectroscopy in silicon. The chip is implemented in a 0.13m SiGe BiCMOS process technology. The transmitter generates and delivers a continuous-wave microwave signal with a frequency range from 895 to 979 MHz and the receiver adopts a direct-conversion architecture. Based on the single-chip tr...

Two commonly used hydrophobic and hydrophilic spin traps for NO, namely Fe2+(DETC)(2)and Fe2+(MGD)(2), respectively, were analyzed via EPR spectroscopy. EPR spectra of trapped NO, together with field position standards, were recorded both in the frozen state and at room temperature. We present a detailed characterization of the EPR spectra of the above paramagnetic NO complexes, concerning g-va...

We have used adenosine diphosphate analogs containing electron paramagnetic resonance (EPR) spin moieties and EPR spectroscopy to show that the nucleotide-binding site of kinesin-family motors closes when the motor.diphosphate complex binds to microtubules. Structural analyses demonstrate that a domain movement in the switch 1 region at the nucleotide site, homologous to domain movements in the...

The application of electron paramagnetic resonance (EPR) spectroscopy in pharmacy of melanin complexes with netilmicin and Cu(II) was presented. The continuous microwave saturation of EPR spectra of DOPA-melanin and the complexes was performed. EPR spectra were measured on an X-band (9.3 GHz) spectrometer at temperatures in the range of 105-300 K. Paramagnetic copper ions decrease the intensity...

Electron paramagnetic resonance (EPR) spectroscopy has been successfully employed to determine radiation dose using alanine. The EPR signal intensity reflects the number of stable free radicals produced, and provides a quantitative measurement of the absorbed dose. The aim of the present study was to explore whether this principle can be extended to provide information on spatial dose distribut...

The photophysical insights of thermally activated delayed fluorescence emitters for OLEDs obtained by time-resolved EPR spectroscopy were summarized.

The interaction between cytochrome P-450,and a spin-labeled analog (l-oxyl-2,2,6,6-tetramethyl-4-piperdinyl nicotinate; RNO) of the inhibitor metyrapone (2-methyl-1,2-di-3-pyridyl-l-propanone) has been studied by both optical absorbance and EPR spectroscopy. RNO was shown to bind to cytochrome P-450,,, competitively with the substrate camphor. In buffer with and without detergent, optical absor...

Three five-coordinate, high spin manganese(ii) "picket fence" porphyrin complexes, [Mn(TpivPP)(L)] (TpivPP = α,α,α,α-tetrakis(o-pivalamidophenyl)porphyrinato; L = 1-MeIm (1-methylimidazole), 1-EtIm (1-ethylimidazole) and 2-MeHIm (2-methylimidazole)), are synthesized and studied by single-crystal X-ray, UV-vis and electronic paramagnetic resonance (EPR) spectroscopy. Structural parameters are in...

The interaction between cytochrome P-450,and a spin-labeled analog (l-oxyl-2,2,6,6-tetramethyl-4-piperdinyl nicotinate; RNO) of the inhibitor metyrapone (2-methyl-1,2-di-3-pyridyl-l-propanone) has been studied by both optical absorbance and EPR spectroscopy. RNO was shown to bind to cytochrome P-450,,, competitively with the substrate camphor. In buffer with and without detergent, optical absor...