Background: E-cadherin is a trans-membrane glycoprotein that plays a critical role in many aspects of cell adhesion as well as establishment and maintenance of epithelial cell polarity. Loss of the adhesive function of E-cadherin seems to promote invasive and metastatic properties of neoplastic cells. Objectives: The present study is a retrospective study aiming to evaluate the loss of E-cadher...

An integral membrane glycoprotein with an apparent molecular weight of 155,000 and isoelectric points ranging from 7.2 to 7.6 has been found to be predominantly expressed on the apical plasma membrane of luminal epithelial cell lining the lobules and terminal ducts in breast. The glyco protein was purified to homogeneity from human milk-fat-globule mem brane and was termed MFGM-gp 155. Polyclon...

Shigella fl exneri causes bacillary dysentery in humans. Essential to the establishment of the disease is the invasion of the colonic epithelial cells. Here we investigated the role of the lipopolysaccharide (LPS) O antigen in the ability of S. fl exneri to adhere to and invade polarized Caco-2 cells. The S. fl exneri 2a O antigen has two preferred chain lengths: a short O antigen (S-OAg) regul...

purpose: to evaluate the results of one 60-degree block conjunctival limbal autograft (clau) surgery in patients with unilateral total limbal stem cell deficiency (lscd). methods: five eyes of 5 patients were included. all patients had a history of chronic total unilateral lscd due to a chemical injury. two patients underwent one 60-degree block clau with amniotic membrane transplantation, two ...

Mucinous cystic neoplasms (MCN) with malignant sarcomatous stroma are rare aggressive tumors and there are few recorded cases. We report a case of MCN that had adenocarcinoma in situ and invasive adenocarcinoma with foci of sarcomatous stroma in a 40-year-old woman. Clear transition from adenocarcinoma areas into sarcomatoid foci was noted. The stromal component showed immunoreactivity for CK7 ...

Secretory meningioma have been described as a distinct variant of meningioma based on their histologic, immunohistochemical and ultrastructural features of epithelial and secretory differentiation of meningothelial cells with accumulation of secretory material in the form of hyaline inclusion. Secretory meningioma is also a benign tumor having similar biological behaviour to that of typical men...

A new monoclonal antibody to epithelial membrane antigen (EMA) E29. A comparison of its immunocytochemical reactivity with polyclonal anti-EMA antibodies and with another monoclonal antibody, HMFG-2. Summary Two polyclonal rabbit antibodies to epithelial membrane antogen (EMA), two mouse monoclonal antibodies (E29 and HMFG-2), and a "cocktail" of these two monoclonals have been compared using a...

Using immunofluorescence techniques, we analyzed the distribution of glycoproteins in normal and keratoconus corneas of humans. Laminin, bullous pemphigoid antigen, fibronectin, and fibrin/fibrinogen were all found in the epithelial basement membrane of normal corneas. Keratoconus corneas produced similar results, except that staining for fibrin/fibrinogen was weak. Fibrin/fibrinogen was absent...

Human milk fat globule membrane, which is said to derive from apical plasma membrane of secretory epithelial cells in breast, was analyzed by sodium dodecyl sulfate-two:dimensional gel electrophoresis. More than 35 components were detected in the gels. One of the major glycoproteins with an apparent molecular weight of 70,000, human milk fat globule membrane glycoprotein, was purified to homoge...

PURPOSE This study examined the potential of a synthetic polymer to support stable epithelial growth when implanted in the feline cornea. METHODS A perfluoropolyether-based polymer was cast into lenticules that were coated with collagen I and implanted in four feline corneas. Epithelial growth onto the lenticules was monitored clinically for 6 weeks, after which time the animals were killed, ...