To explore whether and how T cells can affect myelopoiesis, we investigated myeloid differentiation in a model for T cell-mediated immune activation. We found that CD70-transgenic (CD70TG) mice, which have elevated numbers of interferon-γ (IFN-γ)-producing effector T cells in the periphery and bone marrow, are almost devoid of eosinophilic granulocytes. Induction of allergic airway inflammation...

sputum were stained with hematoxylin-eosin and with a mono¬ clonal antibody against eosinophil cationic protein (anti-EG2; Pharmacia; Uppsala, Sweden) for immunohistochemical evalua¬ tion. Stained mucosal cells were counted according to the method of Moqbel et al,3 and such cells in the sputum were also counted and expressed as a percentage of 500 cells. As shown in Table 1, IPD caused a signif...

BACKGROUND Although nasal polyps are one of the most frequent diseases, their etiopathogenesis remains unclear.Since eosinophils are the main inflammatory cells in the substantial proportion of nasal polyp tissues, they are considered potentially responsible for the etiopathogenesis and prognosis of the disease. Aim of this study was to investigate the relation between mucosal and peripheral eo...

BACKGROUND Eosinophils are effector cells during parasitic infections and allergic responses. However, their contribution to innate immunity has been only recently unravelled. METHODOLOGY/PRINCIPAL FINDINGS Here we show that human eosinophils express CD3 and gammadelta T Cell Receptor (TCR) but not alphabeta TCR. Surface expression of gammadeltaTCR/CD3 is heterogeneous between eosinophil dono...

The lysophospholipids sphingosine 1-phosphate (S1P) and lysophosphatidic acid (LPA) act via G-protein coupled receptors S1P(1-5) and LPA(1-3) respectively, and are implicated in allergy. Eosinophils accumulate at innervating cholinergic nerves in asthma and adhere to nerve cells via intercellular adhesion molecule-1 (ICAM-1). IMR-32 neuroblastoma cells were used as an in vitro cholinergic nerve...

BACKGROUND The objective was to characterise the process and consequences of eosinophil activation and lysis in patients with chronic eosinophilic pneumonia and to compare them with those in patients with eosinophil pulmonary infiltrates from other causes. METHODS Cells from bronchoalveolar lavage fluid of four patients with chronic eosinophilic pneumonia and four patients with eosinophilic i...

Objective/purpose Eosinophil infiltration of gut tissue plays a key role in the pathogenesis of inflammatory bowel diseases (IBD), such as ulcerative colitis. Using a model of allergic asthma, we previously demonstrated that eosinophil migration requires surface expression of the sialomucin CD34, and that Cd34 deletion dampens asthmatic responses in mice. Since CD34 is critical for eosinophil m...

Eosinophils are bi-lobed, multi-functional innate immune cells with diverse cell surface receptors that regulate local and inflammatory responses. Several infectious diseases triggered their build up in the blood tissues. The mobilization of eosinophils into lungs is regulated by a cascade processes guided Th2 cytokine generating T-cells. Recruitment essentially leads to characteristic response...

Immunoglobulin binding on eosinophil surface receptors results in activation of these cells. Evaluating blood eosinophils from atopic subjects, it was investigated whether ligation of immunoglobulin E low-affinity receptor (FcepsilonRII/ CD23) with specific monoclonal antibodies (Mabs) resulted in enhanced eosinophil migration and adhesion molecule expression. Eosinophils from 20 subjects with ...