Aim and objectives: Lead is one of the most important environmental pollutants that plays a significant role in increasing the stability of some other pollutants through changing the microbial profile of soil.  Bacillus subtilis WPI is one of the most abundant bacteria existing in the wastewater. Due to the presence of laccase enzyme in this bacterium, it can facilitate the decomposition proces...

Enzyme immobilization on solid conducting surfaces faces challenges for practical applications in technologies such as biosensors and biofuel cells. Short-term stability, poor electrochemical performance, enzyme inhibition are some unsolved issues. Here, we show a simple methodology bilirubin oxidase (BOD) carbon-based gas diffusion electrode four-electron oxygen reduction reaction. BOD is inco...

TK (transketolase) undergoes inactivation during biocatalytic processes due to oxidation, substrate and product inhibition, reactivity of aldehyde substrates, irreversible inactivation at low pH, and dissociation of cofactors. However, the contribution of protein denaturation to each of these mechanisms is not fully understood. The urea-induced reversible denaturations of the apo- and holo-enzy...

Bioelectrocatalysis using redox enzymes appears as a sustainable way for biosensing, electricity production, or biosynthesis of fine products. Despite advances in the knowledge parameters that drive efficiency enzymatic electrocatalysis, weak stability bioelectrodes prevents large scale development bioelectrocatalysis. In this review, starting from understanding protein instability, we will dis...

much less stable in both cases (Wiseman et al., 1975). Reduced forms contain Fe", which is a high-spin-state form, and this may explain the lower stability, although there is a possibility of a conformational difference for the Fel* form associated with its mechanistic role in oxygen binding. This is another possible case, therefore, of a mechanistic advantage and stability being mutually exclu...

In this paper we assessed the ability of modulators of the activity of glycogen phosphorylase b from the fat body of larval Manduca sexta to stabilize the enzyme against thermal denaturation. This approach has allowed us to distinguish between modulators that stabilize the enzyme, presumably through some conformational effect, from those that do not affect thermal stability. For example, 5'-AMP...