نتایج جستجو برای: enzymatic lyses

تعداد نتایج: 55202  

پایان نامه :وزارت علوم، تحقیقات و فناوری - دانشگاه شیراز - دانشکده علوم پایه 1391

a simple, rapid and low-cost scanner spectroscopy method for the glucose determination by utilizing glucose oxidase and cdte/tga quantum dots as chromoionophore has been described. the detection was based on the combination of the glucose enzymatic reaction and the quenching effect of h2o2 on the cdte quantum dots (qds) photoluminescence.in this study glucose was determined by utilizing glucose...

Journal: :jundishapur journal of microbiology 0
shabnam sadoogh abbasian molecular and medicine research center, arak university of medical sciences, arak, ir iran ehsanollah ghaznavi rad department of microbiology and immunology, school of medicine, arak university of medical sciences, arak, ir iran neda akbari department of microbiology, faculty of science, arak branch, islamic azad university, arak, ir iran mohammad reza zolfaghari department of microbiology, qom branch, islamic azad university, qom, ir iran iraj pakzad clinical microbiology research center, ilam university of medical sciences, ilam, ir iran hamid abtahi molecular and medicine research center, arak university of medical sciences, arak, ir iran; molecular and medicine research center, arak university of medical sciences, arak, ir iran. tel: +98-8612227109, fax: +98-8612227136

materials and methods the expression of hyaluronidase antigenic fragments was optimized using iptg concentration, time of induction, temperature, culture, and absorbance of 0.6-0.8-1 at 600 nm. afterwards, the expressed proteins were purified and the enzymatic activity was assessed by turbid metric method. results data indicated that maximum protein is produced in od = 0.8, 0.5 mm isopropyl β-d...

Journal: :journal of the iranian chemical research 0
mohammad taghi taghizadeh department of physical chemistry, faculty of chemistry, university of tabriz, tabriz, iran. zahra abbasi department of physical chemistry, faculty of chemistry, university of tabriz, tabriz, iran.

thermoplastic starch (tps) materials present several advantages to the plastic industry andwhen blended with other materials they can exhibit improved mechanical and moisturesensitivity properties compared to pure tps materials. further investigations on tps: pvablends are of particular interest due to their excellent compatibility and improved properties suchas tensile strength, elongation, to...

Journal: :international journal of environmental research 0
e. mobarak-qamsari department of biology, faculty of science, alzahra university, tehran, iran r. kasra-kermanshahi department of biology, faculty of science, alzahra university, tehran, iran m. nosrati biotechnology group, chemical engineering department, tarbiat modares university, tehran, iran t. amani department of chemical engineering, faculty of engineering, university of kurdistan, sanandaj, iran

enzymatic extract preparation from pseudomonas aeruginosa km110 under accession no. hq730879 with lipase activity (0.3 u/ml), was used to perform enzymatic hydrolysis pretreatment of a synthetic dairy wastewater with 1000 mg/l total fat content. the pretreatment was optimized for 48 h hydrolysis time, at 45æ% c with 10% v/v enzymatic extract. the biological treatment of synthetic dairy wastewat...

Journal: :پژوهش های علوم و صنایع غذایی ایران 0
h.r. khazaei m. nasiri mahallati m. j. arshadi

in order to estimate the reducing sugar in tubers of potato by using a manual glucose determiner, an experiment was conducted in factorial arrangement based on completely randomized design with three replications. in this research reducing sugar of four cultivars of potato – agria, sante, with two methods: enzymatic and a manual glucose determiner. the results picaso and kizer – that maintained...

Journal: :Proceedings of the National Academy of Sciences of the United States of America 2017
William H Conrad Morwan M Osman Jonathan K Shanahan Frances Chu Kevin K Takaki James Cameron Digby Hopkinson-Woolley Roland Brosch Lalita Ramakrishnan

Mycobacterium tuberculosis and Mycobacterium marinum are thought to exert virulence, in part, through their ability to lyse host cell membranes. The type VII secretion system ESX-1 [6-kDa early secretory antigenic target (ESAT-6) secretion system 1] is required for both virulence and host cell membrane lysis. Both activities are attributed to the pore-forming activity of the ESX-1-secreted subs...

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A fermentation process for production of super coiled plasmid DNA in E. coli for use as a DNA vaccine was developed. In the process a feed-back control nutrient feeding strategy based on pH and DO was used to regulate the cell growth rate by controlling the interactivity of the nutrient feed rate, pH and DO. The process increased the total yield of plasmid DNA by approximately 4 fold as compare...

Journal: :The Journal of Experimental Medicine 1993
A Molloy P A Meyn K D Smith G Kaplan

We have established a long-term culture system to study macrophages chronically infected with mycobacteria. Monocytes are infected with Bacillus Calmette-Guerin (BCG) and support exponential intracellular replication without profound perturbation of normal host cell function. We have used this system to investigate lymphokine-activated killer (LAK)-mediated cytolysis. We have found that interle...

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