Hen egg white lysozyme, fully inactivated by the affinitylabeling reagent, the 2’,3’-epoxypropyl fl-glycoside of di-(NaCetyl-D-glUCOSamine), contained 1 mole of the label per mole of protein. Total enzymatic digestion of the reduced and carboxymethylated affinity-labeled enzyme afforded, as a major radioactive product, a compound composed solely of aspartic acid and glucosamine at the molar rat...

The Wharton's Jelly (WJ) is an established source of mesenchymal stem cells (MSC). We compared 3 methods of extracting WJ-MSC from cryopreserved tissue and determined that enzymatic digestion of the WJ yielded the most viable MSC, compared to the explant and mechanical digestion methods. The enzymatically-released WJ-MSC conformed to the International Society for Cellular Therapy (ISCT) criteri...

Enzymatic activities for digestion of proteins and carbohydrates were compared among three organs of the digestive system of Pimelodus maculatus in two reservoirs with different trophic conditions during the winter of 2006. The aim was to test the hypothesis that enzymatic activity for the digestion of proteins and carbohydrates differed among organs and that such activities differ between the ...

Slowly digestible starches have received interest due to their lower increase of postprandial blood glucose and insulin levels and, hence, modification towards slower digestibility has commercial interest. However, chemical characteristics driving enzymatic (digestive) degradation are not fully unraveled. The digestion properties been linked crystalline type, chain length distribution, amylose ...

Murine and human esophageal myofibroblasts are generated via enzymatic digestion. Neonate (8-12 day old) murine esophagus is harvested, minced, washed, and subjected to enzymatic digestion with collagenase and dispase for 25 min. Human esophageal resection specimens are stripped of muscularis propria and adventitia and the remaining mucosa is minced, and subjected to enzymatic digestion with co...

The field of proteomics has emerged as a valuable analytical tool for elucidating cellular and biological systems at the molecular level. As there is an ever-growing demand for new highly automated, high-throughput, and sensitive analytical tools, a key challenge is the characterization of lowabundance proteins that are crucial in modulating biological functions of cells and may also be associa...

Our protocol describes adoptive transfer of antigen presenting cells (APCs) isolated from the lungs by enzymatic digestion and magnetic enrichment. This protocol can be used to study APC functions and trafficking.

background and objective: the outer membrane protein w ( ompw ) of vibrio cholerae is involved in stimulating the im- mune response via induction of protective immunity. it also plays an important role in bacterial pathogenesis by increasing the adaptability of pathogenic strains. in this study we aimed to clone v. cholerae ompw gene in the strain x-33 of pichia pastoris. materials and methods:...

background: the aim of this study was to clone pparγ1 cdna in an appropriate mammalian expression vector, with a chimeric cdna form, encompassing pparγ with enhanced green fluorescent protein (egfp) cdna. this recombinant plasmid will be used for further analyses to investigate the molecular mechanism of pparγ1 for neural differentiation process. moreover, the nuclear localization of the pparγ1...

in biological wastewater treatment large quantities of sludge are produced. this sludge is highly putrescible.for disposal and safe usage should be stabilized. the anaerobic digestion is a standard technique for stabilization. due to the rate imiting step of sludge hydrolysis, anaerobic degradation is a slow process with high digestion time. due to high retention time and sludge dewatering prob...