This experiment was studied to examine the in vitro developmental potential of enucleateddomestic cat oocytes reconstructed with skin fibroblasts from female domestic and leopard cats. The cumulus oocyte complex of domestic cat were cultured in IVM medium under 38.5°C, 5%CO2, 5%O2,90%N2 for 24 hours. The matured oocytes were enucleated and individual donor cell was inserted into...

PURPOSE To date, the parameters for evaluating enucleation efficiency have only considered enucleation time, although operators simultaneously consume both time and energy during holmium laser enucleation of the prostate. This study was undertaken to find a better way of assessing enucleation skills, considering both enucleation time and consumed energy. MATERIALS AND METHODS One hundred (n=1...

Prostaglandin E1 and the beta-adrenergic hormone l-isoproterenol stimulated cyclic AMP formation in both nucleated and enucleated myeloid leukemic cells that could be induced to differentiate normally to mature cells by the macrophage- and granulocyte-inducing protein MGI (MGI+D+ cells). Enucleated as well as nucleated MGI+D+ cells also desensitized to these hormones, indicating that this desen...

The objective of this paper was to study the characteristics of closed-loop smooth pursuit eye movements of 15 unilaterally eye enucleated individuals and 18 age-matched controls and to compare them to their performance in two tests of motion perception: relative motion and motion coherence. The relative motion test used a brief (150 ms) small stimulus with a continuously present fixation targe...

Many curated databases are constructed by scientists integrating various existing data sources. Most current approaches to provenance in databases are based on views and fail to take account of the added value of the work done by scientists in manually creating and modifying data. Capturing provenance in such an environment is a challenging problem, requiring changes in practice, changes to exi...

Previous research has shown that observers with early unilateral enucleation have selectively better sensitivity to luminance contrast than monocular viewing controls [González et al., 2002; Vision Research 36 (1) (1996) 175; Vision Research 36 (1996) 3011; Vision Research 37 (17) (1997) 2465]. We asked whether unilateral enucleation specifically enhances all levels of luminance processing. Enu...

We previously established a novel method for nuclear transfer in medaka (Oryzias latipes) using non-enucleated, diploidized eggs as recipients for adult somatic cell nuclei. Here we report the first attempt to apply this method to another fish species. To examine suitability of using non-enucleated eggs as recipients for nuclear transfer in the zebrafish (Danio rerio), we transferred blastula c...