Human enterotoxigenic Escherichia coli isolates expressing the colonization factor antigen CFA/IV (previously designated PCF8775) produce plasmid-encoded CS5 fimbriae. The nucleotide sequence of the region encoding the major CS5 fimbrial subunit was determined. The subunit is synthesized as a precursor of 203 amino acids (20.85 kDa) with a mature protein of 181 amino acids corresponding to a si...

Policy interest since the early 1980s has focused in different ways on the creation of a large, productive, taxable economy – in which entrepreneurship plays a role for employment, income growth and innovation. The current understanding of various forms of entrepreneurship remains incomplete, focusing largely on productive and unproductive entrepreneurship. However, destructive entrepreneurship...

Porcine neonatal diarrhea and postweaning diarrhea caused by enterotoxigenic Escherichia coli (ETEC) result in significant morbidity and mortality and are economically important diseases of pigs.1 Secretory diarrhea associated with ETEC infection is mediated by any of several enterotoxins, including heat labile enterotoxin (LT), heat-stable enterotoxin-a (STa), and heat-stable enterotoxin-b (ST...

Clostridium difficile and enterotoxigenic C. perfringens are significant causes of enterocolitis in horses. Based on detection of specific clostridial toxins in fecal samples, C. difficile was associated with 22% and 17% of cases of colitis in adult horses and foals, respectively. Enterotoxigenic C. perfringens was associated with 19% and 29% of cases in adult horses and foals. Incorporation of...

Enterotoxigenic Escherichia coli grown in the presence of lincomycin and tetracycline produced an increased amount of heat-labile enterotoxin (LT). These antibiotics increased the production of not only extracellular LT but also intracellular LT. On the other hand, lincomycin did not stimulate the production of heat-stable enterotoxin by enterotoxigenic E. coli. The extracellular LTs produced i...

The present study describes a colony hybridization setup for identification of enterotoxigenic Escherichia coli obviating the need for advanced equipment and radioactive isotopes. With a modest laboratory arrangement, polynucleotide gene probes were produced in large quantities. The probes were labeled with digoxigenin and, after hybridization, detected with an antidigoxigenin alkaline phosphat...

Most lung tumor linkage studies focus on identifying loci that confer susceptibility or resistance irrespective of the tumor types developed. However, different mouse strains develop different types of lung tumors. A major obstacle for genetic studies of these differences is the lack of reproducible, quantitative, and uniform assessment of tumor type. We have previously described a new variable...

T regulatory cells trigger an oncogenic immune response against enterotoxigenic B. fragilis infection. The implications of an overall shift in the colonic homeostasis are discussed.