Whole-cell protein analysis was performed for differentiating 150 enterococcal isolates to the species level, which had previously been identified by extended phenotypic conventional tests. Whole-cell protein profile (WCPP) showed a high degree of similarity within species and comparison between species revealed important differences in band profiles. All Enterococcus faecalis and Enterococcus ...

Enterococcus faecalis and Enterococcus faecium infections are increasingly difficult to treat due to high levels of resistance to antibiotics. PlyV12, a bacteriophage lytic enzyme, was isolated and shown to effectively kill both E. faecalis and E. faecium (including vancomycin-resistant strains), as well as other human pathogens. We propose its development and use as an alternative therapeutic ...

OBJECTIVE to investigate the prevalence and antimicrobial resistance of Enterococcus species isolated from a university hospital, and explore the mechanisms underlying the antimicrobial resistance, so as to provide clinical evidence for the inappropriate clinical use of antimicrobial agents and the control and prevention of enterococcal infections. METHODS a total of 1,157 enterococcal strain...

The ability to colonize patients successfully may be essential for the emergence and spread of resistant nosocomial strains. We determined the presence of Esp, a surface protein involved in colonization ability in Enterococcus faecalis, in 96 Enterococcus faecium isolates from hospitalized patients (77 PFGE clones), 33 faecal isolates from healthy volunteers (32 clones) and 20 environmental iso...

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microorganisms play a major role in the initiation and perpetuation of pulpal and periapical disease. in order to predictably achieve a bacteria-free root canal system, it is necessary to use intracanal medicaments. calcium hydroxide is the most common intracanal medicaments. it is effective against primary infections. however, its effectiveness against enterococcus faecalis and candida albican...

introduction: the aim of this study was to compare the antimicrobial effects of 2.5% sodium hypochlorite (naocl), 2% chlorhexidine gluconate (chx) and biopure mtad (mtad) on enterococcus (e) faecalis -contaminated root canals of human extracted teeth. materials and methods: seventy human intact extracted single-rooted teeth with straight root canal randomly divided into 5 groups: positive contr...

conclusions according to these findings, antibiotic-resistance patterns have changed, and vancomycin resistance, especially among e. faecium, is rising because of nosocomial infections. consequently, it has become a serious subject for patients admitted into a hospital. results a total of 53 enterococci were isolated from clinical samples of blood, urine, wounds, sputum, and cerebrospinal fluid...

Most Enterococcus faecalis and E. faecium are harmless to humans; however, strains harboring virulence genes, including esp, gelE, cylA, asa1, and hyl, have been associated with human infections. E. faecalis and E. faecium are present in beach waters worldwide, yet little is known about their virulence potential. Here, multiplex PCR was used to compare the distribution of virulence genes among ...

The presence, distribution and expression of cassette chromosome recombinase (ccr) genes, which are homologous to the staphylococcal ccrAB genes and are designated ccrAB(Ent) genes, were examined in enterococcal isolates (n=421) representing 13 different species. A total of 118 (28 %) isolates were positive for ccrAB(Ent) genes by PCR, and a number of these were confirmed by Southern hybridizat...