A method for selective and comprehensive enrichment of N-linked glycopeptides was developed to facilitate detection of micro-heterogeneity of N-glycosylation. The method takes advantage of the inherent properties of Fbs1, which functions within the ubiquitin-mediated degradation system to recognize the common core pentasaccharide motif (Man3GlcNAc2) of N-linked glycoproteins. We show that Fbs1 ...

The objective of this study was to compare the diagnostic performance of a direct isolation method for Erysipelothrix spp. with a broth-based enrichment technique. Samples were obtained from three sources: (1) experimentally inoculated pigs, (2) porcine tissue samples submitted to the Iowa State University Veterinary Diagnostic Laboratory, and (3) tissues from condemned carcasses at an abattoir...

Vibrio parahaemolyticus is a marine bacterium with a worldwide distribution and is frequently associated with human outbreaks of infection. Detection and isolation of V. parahaemolyticus from natural sources is often problematical because of limitations in the analytical procedures. We evaluated a combination of conventional and molecular protocols previously described for the investigation of ...

A method is introduced for quantitating protein synthetic rates in humans by use of (2)H(2)O. Its validity was tested in subjects with end-stage renal disease. Six clinically stable subjects, hemodialyzed three times weekly, ingested (2)H(2)O to a body water (2)H enrichment of approximately 0.4%. On dialysis, body water enrichment declined to approximately 0.1%. Enrichment of the alpha-hydrogen...

We construct a nonconforming virtual element method (ncVEM) based on approximation spaces that are enriched with special singular functions. This ncVEM is tailored for the of solutions to elliptic problems, which have singularities due geometry domain. Differently from traditional extended Galerkin approach, enrichment local functions, no partition unity employed. Rather, design hinges upon str...