Lipid-derived free radicals were detected by electron paramagnetic resonance (EPR) spectrometry when cultured endothelial cells attached to Cytodex beads were exposed to iron-induced oxidant stress in the presence of the spin trap alpha-(4-pyridyl-1-oxide)-N-tert-butylnitrone (POBN). Radical adduct formation was enhanced greatly when the cells were supplemented during growth with polyunsaturate...

Using a new superfusion procedure in vitro, we have previously reported that the NMDA-evoked release of newly synthesized 3H-dopamine (DA) was higher in matrix- than in striosome-enriched areas of the rat striatum. In addition, GABAergic medium-sized spiny neurons were shown to be indirectly involved in this regulation. Since dynorphin and GABA are colocalized in a population of medium-sized sp...

Beta-carotene is a valuable carotenoid in high demand as a natural food coloring agent, provitamin A, additive to cosmetics, and health food. It can be accumulated up to more than 10% of cellular dry weight of Dunaliella salina under carotenogenic conditions such as high irradiance, high temperature, high salt concentration and nutrient deficiency. High beta-carotene productivity in Dunaliella ...

Plant growth regulators (PGRs) have an important role in callus induction and organogenesis of plant explants cultured in vitro conditions. Callus has a proper potential for plant regeneration through indirect organogenesis and embryogenesis as well suspension culture, genetic transformation and production of secondary metabolites. In current experiment, leaf explants of Petunia × hybrida F1 we...

A stochastic model of the chemical enrichment of metal-poor systems by core-collapse (Type II) supernovae is presented, allowing for large-scale mixing of the enriched material by turbulent motions and cloud collisions in the interstellar medium. Infall of pristine material is taken into account by following the evolution of the gas density in the medium. Analytical expressions were derived for...