background endophytic fungi, which have been reported in numerous plant species, are important components of the forest community and contribute significantly to the diversity of natural ecosystems. objectives the current study aimed to evaluate and characterize, at the molecular level, the diversity and antimicrobial activities of endophytic fungi from medicinal plants in saudi arabia. materia...

The conversionof carboxyhemoglobin(H bCO) to cyanmethemoglobin (M HbCN) by Drabkin’s reagent has been shown to be considerablyslower than is the same reaction with oxyhemoglobin(Hb02). Conversionof Hb02 to MHbCN is complete in 10 mm. whereas total conversionof HbCO requires 90-120 mm. This difference in reaction rate must be consideredin the determination of total hemoglobinas MHbCN when the bl...

A cellulolytic, thermophilic actinomycete (previously isolated from municipal refuse compost samples) was identified as Thermomonospora curvata. A determination was made of the optimal conditions for cellulase production by T. curvata when grown at 55 C in a medium containing mineral salts, cellulose, and yeast extract. The pH and temperature optima (pH 6.0 and 65 C) for the cellulase produced ...

The endophytic bacterial microbiome, with an emerging role in plant nutrient acquisition and stress tolerance, is much less studied in natural plant populations than in agricultural crops. In a previous study, we found consistent associations between trees in the pine family and acetic acid bacteria (AAB) occurring at high relative abundance inside their needles. Our objective here was to deter...

This work aimed a survey on the biodiversity of maize endophytic actinomycete, and an evaluation of their potential to control the phytopathogenic fungi. From several regions of São Paulo state, 40 strains were isolated from the healthy maize plants. The identification of these strains, based on morphological properties and fatty acid methyl ester (FAME) profile showed that most of them belonge...

The endophytic actinobacterial population in the roots of wheat grown in three different soils obtained from the southeast part of South Australia was investigated by terminal restriction fragment length polymorphism (T-RFLP) analysis of the amplified 16S rRNA genes. A new, validated approach was applied to the TRFLP analysis in order to estimate, to the genus level, the actinobacterial populat...

Actinomycetes are involved in important environmental processes such as the decomposition of organic matter. In this study, we examined the impact of crop rotation on the actinomycetes community colonizing rice straw residues in soil over 3 field seasons by means of Denaturing Gradient Gel Electrophoresis (DGGE) fingerprinting analysis of actinomycetes 16S rRNA gene fragments amplified from fie...