The development of new seedless cultivar represents one the most important goals in table grape breeding programmes worldwide. common technique to obtain cultivars is embryo rescue, an approach that allows isolation immature embryos and their cultivation vitro. In this study, a total 23 crosses (developed employing seeded parent) were performed during two seasons (2017 2018) for 1140 seedlings....

Introgression is the movement of genes or gene flow from one species into the gene pool. wide hybridisation breaks the species barrier for gene transfer and makes it possible to transfer the genome of one species to other, which results alteration in genotypes and phenotypes of the progeny. The main objective of wide hybridisation is to transfer desirable genes, to create genetic variability wh...

Rapid endocytosis of lipoprotein receptors involves NPxY signals contained in their cytoplasmic tails. Several proteins, including ARH and Dab2, can bind these sequences, but their importance for endocytosis may vary in different cell types. The lipoprotein receptor megalin is expressed in the visceral endoderm (VE), a polarized epithelium that supplies maternal nutrients to the early mammalian...

During morphogenesis, forces generated by cells are coordinated and channeled by the viscoelastic properties of the embryo. Microtubules and F-actin are considered to be two of the most important structural elements within living cells accounting for both force production and mechanical stiffness. In this paper, we investigate the contribution of microtubules to the stiffness of converging and ...

A case is reported of a patient with critically low anti-mullerian hormone (AMH) having successful 'rescue' oocyte retrieval in natural in vitro fertilization (IVF) cycle 24 hours after the initial procedure had resulted in no oocyte being collected. Following administration of 10,000 units human chorionic hormone (hCG) a second retrieval was performed 24 hours after the first, producing one Me...

Citrus rootstock breeding is important to improve their resistance diseases, pests and adverse environmental conditions. The majority of citrus species have nuceller embryony. Although Clementine mandarin monoembryonic, in some cases (intercrossing etc.) abortive seed formation can be seen frequently. In this study, × Carrizo citrange were crossed. the interspecies hybridization (Citrus Ponciru...

The maternal D-raf serine/threonine kinase acts downstream of Torso (Tor) for specification of cell fates at the embryonic termini. D-raf activity is also required in other signal transduction pathways and consistent with its pleiotropic role, we find accumulation of a 90-kD D-raf protein throughout embryonic development. We also characterize the accumulation of maternal D-raf proteins in 0-2-h...

In an attempt to determine whether the rescue of developing motoneurons (MNS) from programmed cell death (PCD) in the chick embryo following reductions in neuromuscular function involves muscle or neuronal nicotinic acetylcholine receptors (nAChRs), we have employed a novel cone snail toxin alphaA-OIVA that acts selectively to antagonize the embryonic/fetal form of muscle nAChRs. The results de...