Reactive oxygen species (ROS) promote the germination of several seeds, and antioxidants suppress it. However, questions remain regarding the role and production mechanism of ROS in seed germination. Here, we focused on NADPH oxidases, which produce ROS. After imbibition, NADPH oxidase mRNAs were expressed in the embryo and in aleurone cells of barley seed; these expression sites were consisten...

Phytochromes phyB and phyA mediate a remarkable developmental switch whereby, early upon seed imbibition, canopy light prevents phyB-dependent germination, whereas later on, it stimulates phyA-dependent germination. Using a seed coat bedding assay where the growth of dissected embryos is monitored under the influence of dissected endosperm, allowing combinatorial use of mutant embryos and endos...

When applied to young nondormant embryos of sunflower (Hellanthus annus) (7-10 day[s] after pollination [DAP]), abscisic acid (ABA) inhibited germination as long as it was present. However, whatever the dose used and the duration of its application, ABA was unable to induce dormancy because after transfer of treated embryos to control (without ABA) medium, germination occurred. Thereafter, exog...

Immunolocalization of mannans in the seeds of Brachypodium distachyon reveals the presence of these polysaccharides in the root embryo and in the coleorhiza in the early stages of germination (12h), decreasing thereafter to the point of being hardly detected at 27h. Concurrently, the activity of endo-β-mannanases (MANs; EC 3.2.1.78) that catalyse the hydrolysis of β-1,4 bonds in mannan polymers...

Freshly harvested seed of #1339-1-5, a selection miiade by the late C. H. MYERS, from Wisconsin Hollander, and various strains of Glory cabbage exhibit a pronounced dormancy and uneven germination, thus extending the breeding cycle from 1 to 2 years. This type of dormancy gradually disappears during several weeks of air-dry storage at room temperature, but by this time it is too late for field ...

abstract in this experiment the effects of two type of medium cultures, gibberellic acid and dark/cold and light/warm culture conditions on mature embryos germination of a local cultivar of walnut were investigated. the mature fruits were collected at 22 weeks postanthesis and after disinfection the mature embryo along with cotyledon pieces were excised and then were cultured on mt and modified...

There is a paucity of information in the literature concerning the metabolic changes that occur in the endosperm, scutellum, and embryonic axis during the germination of corn. The work of Toole (20) constitutes a major exception, although this work was more concerned with morphological changes during germination than with changes in chemical constituents and metabolism. Although seed germinatio...

Seed germination is a complicated biological process that requires regulation through various enzymatic and non-enzymatic mechanisms. Although it has been recognized that reactive oxygen species (ROS) regulate radicle emergence and root elongation in a non-enzymatic manner during dicot seed germination, the role of ROS in monocot seed germination remains unknown. NADPH oxidases (NOXs) are the m...

RNA metabolism of embryo and aleurone of barley grains (Hordeum vulgare L. cv. Himalaya) was studied to elucidate the role of these tissues in the control of alpha-amylase synthesis and germination. The extent of (3)H-uridine incorporated into various RNA classes of the embryo during the first 12 hours of germination was low but constant. Subsequently, there was a rapid increase in RNA synthesi...