The issue of fertility preservation in patients with cervical cancer is getting more and common considering the improved effectiveness early diagnosis treatment cancer. There a number evidence-based tactics available to diagnosed These have been proved effective include methods such as ovarian transposition; oocyte, embryo tissue cryopreservation. Nonetheless, there are no existing medical algo...

Background: Embryo cryopreservation is an important part of assisted reproductive technologies to increase cumulative pregnancy rate in clients. However, clinical settings, embryos can be subjected repeated cryopreservation-warming cycles due certain circumstances. There are limited data on the effect such may have success embryo transfer procedures. In this study, we investigated cleavage-stag...

Ovarian stimulation followed by oocyte aspiration has facilitated a number of cryopreservation techniques. Embryo cryopreservation has become a well-established and highly endorsed form of fertility preservation in young female cancer patients (American Society for Reproductive Medicine, American Society of Clinical Oncology, European Society of Human Reproduction and Embryology, British Fertil...

A slow freezing/rapid thawing method for the cryopreservation of human oocytes has been employed using a sodium-depleted culture media. In 53 frozen egg-embryo transfer (FEET) cycles, a 60.4% survival rate post-thaw was obtained and a 62.0% fertilization rate following intracytoplasmic sperm injection. Overall pregnancy rates were 26.4% per thaw attempt, 30.4% per patient, and 32.6% per embryo ...

Embryo cryopreservation is a vital part of any assisted conception programme. We analysed our experience with respect to various clinical factors which may influence success and help modify our own practice. Factors studied were endometrial preparation, number of embryos transferred, woman's age at embryo freezing and endometrial thickness. The 212 cycles analysed represented all our frozen emb...

OBJECTIVE To characterize the early experience of a clinical program designed to provide strategies for fertility preservation to female cancer patients about to undergo chemotherapy or radiation therapy. DESIGN Retrospective chart review; case-control study. SETTING Academic medical center. PATIENT(S) Sixty-five female cancer patients and 57 age-matched infertility patients. INTERVENTI...

Cryopreservation is a key technology in biology and clinical practice. This paper presents a digital microfluidic device that automates sample preparation for mammalian embryo vitrification. Individual micro droplets manipulated on the microfluidic device were used as micro-vessels to transport a single mouse embryo through a complete vitrification procedure. Advantages of this approach, compar...

Four years ago, the results of an experimental study led us to conclude that in the mouse, cryopreservation of preimplantation embryo induced post-natal effects and thus could not be considered as neutral for the long-term development (Dulioust et al., 1995). This raised some concern in the scientific community and in the public about the safety of embryo cryopreservation in human assisted proc...

The purpose of this research was to evaluate three embryo biopsy techniques used for preimplantation genetic diagnosis (PGD) in cattle and to recommend the least invasive one for current use, especially when PGD is followed by embryo cryopreservation. Three hundred bovine embryos were biopsied by either one of the needle, aspiration or microblade method, and then checked for viability by freezi...

Cryopreservation of Senegalese sole sperm can represent an alternative to overcome some reproductive problems of this species. However, it is important to guarantee the safe use of cryopreserved sperm by selecting an appropriate protocol according to a high demand quality need to be ensured. It has been demonstrated that traditional assays such as motility and viability do not provide enough in...