Ethidium monoazide (EMA) and propidium monoazide (PMA) have been utilized for selective PCR amplification of DNA from viable bacterial cells. In this study, we compared the abilities of EMA and PMA, together with real-time PCR, to specifically distinguish dead Legionella cells from viable cells. Several experiments showed that PMA or EMA treatment could specifically prevent the PCR amplificatio...

BACKGROUND Ecological momentary assessment (EMA) uses real-time data collection to assess participants' behaviors and environments. This paper explores the strengths and limitations of using EMA to examine social and environmental exposure to tobacco in urban India among older adolescents and adults. OBJECTIVE Objectives of this study were (1) to describe the methods used in an EMA study of t...

This paper presents a hardware-efficient memory allocation (EMA) technique designed to eliminate both internal and external fragmentation that appear in the buddy system. EMA can allocate a free memory block of any size in any part of memory. Hardware implementation of EMA is introduced, but only part of its circuits is shown in the paper due to the space limitation. Simulation results show tha...

We detected Legionella species in 111 bath water samples and 95 cooling tower water samples by using a combination of conventional plate culture, quantitative polymerase chain reaction (qPCR) and qPCR combined with ethidium monoazide treatment (EMA-qPCR) methods. In the case of bath water samples, Legionella spp. were detected in 30 samples by plate culture, in 85 samples by qPCR, and in 49 sam...

BACKGROUND AND METHODOLOGY This survey was undertaken to obtain the opinions of women in the UK about their home management of early medical abortion (EMA) with mifepristone and misoprostol. All eligible women undergoing EMA at any BPAS clinic during a 2-week period were invited to participate. Women were contacted by telephone 1 week after the EMA and a five-item structured questionnaire was a...

This study examines (1) the effect of environmental strategy and Environmental Management Accounting (EMA) activity management decision quality; (2) EMA (3) quality corporate performance, performance on companies with ISO 14001 certifications in Indonesia. We used online surveys to reach managers from that received Indonesia Stock Exchange. analyzed data 54 respondents using Partial Least Squar...

In recent years, increasing numbers of human campylobacteriosis cases caused by contaminated water have been reported. As the culture-based detection of Campylobacter is time consuming and can yield false-negative results, the suitability of a quantitative real-time PCR method in combination with an ethidium monoazide pretreatment of samples (EMA-qPCR) for the rapid, quantitative detection of v...

Clinical data sharing for data transparency has potential to strengthen academic research, the practice of medicine and the integrity of clinical trial systems. This topic is becoming popular nowadays amongst leading pharmaceutical companies, and it raises concerns around the potential leak of personal health information of patients participating in clinical trials. The European Medicines Agenc...

OBJECTIVE The objective of this study was to determine the prevalence of celiac disease (CD) in adults with autoimmune thyroid disease (ATD) from the endocrinology outpatient setting in a university hospital in Southern Brazil. SUBJECTS AND METHODS From the years 2007 to 2011, 254 patients with ATD were enrolled consecutively, Grave's disease was diagnosed in 143 (56.3%) and Hashimoto's thyro...

The association of celiac disease and type I diabetes mellitus has been known for some time. This study was undertaken to investigate the prevalence of celiac disease (CD) in diabetic children and adolescents. Eighty-seven patients (44 females, 43 males) aged 218 years, with type I diabetes participated in this study. A group of 87 healthy unrelated girls and boys matched for age and gender ser...