phage elisa is a common method to confirm binding of obtained phages from phage display technique to related antigens. enzyme-conjugated antibody directed against the major capsid protein (pviii) or enzyme-conjugated secondary antibody against the primary antibody is used as a detection system in phage elisa. we have suggested to express an acid phosphatase (sapm) enzyme on m13 pix minor coat p...

Background and objectives: Cutaneous leishmaniasis is endemic in most areas of Iran, and the diagnosis of its species is essential for controlling the disease. Leishmania major is the causative agent for cutaneous leishmaniasis in humans. Molecular methods are generally more sensitive than microscopic methods. The present study aimed to use a polymerase chain reaction-enzyme linked immunosorben...

The enzyme-linked immunosorbent assay (ELISA) technique is the backbone of the diagnostic assay for detection of infectious and allergic diseases. Here, we demonstrate a unique ELISA method for the detection of an antigen or antibody, in which ELISA steps are carried out under pressure instead of conventional thermal incubation. Pressure-mediated ELISA (PELISA), carried out in 1 h shows more th...

Hepatitis B virus (HBV) DNA diagnosis plays a critical role in the transmission of HBV infections. Accurate protocols detection will reduce spread from blood donors. Therefore, this research compared detecting potentials three different detection. Blood 200 donors at University college bank Ibadan were sampled and tested for using ABON™ HBsAg Surface Antigen Tests (strip test), Monolisa TM ULTR...

A new immunologically based flow cytometry (IFCM) technique was developed to enumerate Aureococcus anophagefferens, a small pelagophyte alga that is the cause of "brown tides" in bays and estuaries of the mid-Atlantic states along the U.S. coast. The method utilizes a monoclonal antibody conjugated to fluorescein isothiocyanate (FITC-MAb) to label the surface of A. anophagefferens cells which a...

Traditional geostatistical space-time geostatistics is not able to take account of the generally strongly non-linear dynamics of multivariate space-time processes. To this effect physico-chemical transport models are in general more suitable. However, as the latter do not fully master the complexity of the processes they attempt to describe, either because of simplifying hypotheses or because t...

In this paper, we present new attacks on the redactable signature scheme introduced by Kundu and Bertino at VLDB ’08. This extends the work done by Brzuska et al. at ACNS ’10 and Samelin et al. at ISPEC ’12. The attacks address unforgeability, transparency and privacy. Based on the ideas of Kundu and Bertino, we introduce a new provably secure construction. The corresponding security model is m...

Diarrhoea caused by Cryptosporidium is usually mild in immune competent individuals but severe in the young and those with underlying disease leading to compromised immunity. The conventional diagnosis of Cryptosporidium requires observation of the infective oocysts however, their tiny size yields indistinct results, thus limiting the effectiveness of the conventional diagnostic technique, modi...

Isolates of herpes simplex virus which had previously been typed by restriction enzyme analysis were typed again with a commercial ELISA system using polyclonal antibodies. There was complete correlation between the two techniques. Although restriction is more precise and definitive, when typing only is required the simplicity of ELISA makes it the preferred technique.

results the optimal conditions were considered to perform elisa. comparison between results obtained from optimized elisa kit and commercial elisa kit showed a good agreement. background haemophilus influenzae type b (hib) infection has high morbidity and mortality rate, especially in children under 5 years of age. enzyme-linked immunosorbent assay (elisa) technique is the most used method to d...