Abstract The objective of this work was to evaluate microfluidic chip electrophoresis, known as lab-on-a-chip technique, for the detection milk adulteration using cheese whey in comparison with SDS-PAGE. Raw, pasteurized, processed at an ultra-high temperature (UHT), and powdered samples received increasing concentrations (0, 1, 2.5, 5, 10, 20, 30, 50, 100% v/v), were subjected electrophoresis ...

To detect the genome of viruses (in environmental and clinical samples), we use electrophoresis running buffer after PCR reaction. Also, buffers were used widely to separate any DNA molecule. In this paper, four types previously known compare which is easy for preparation, simple in structure, low cost good performance agarose gel electrophoresis. For this, two concentration (1%, 2%) ladder (10...

Two steps of enzymatic oxidations from hypoxanthine to uric acid with xanthine oxidase (XOD) were kinetically analyzed by capillary electrophoresis/dynamic frontal analysis. When a substrate solution was introduced into separation buffer containing XOD, the reaction continuously proceeded during electrophoresis and product resolved zone. A plateau signal detected based on constant rate XOD. The...

Forensic DNA analysis has vastly evolved since the first forensic samples were evaluated by restriction fragment length polymorphism (RFLP). Methodologies advanced from gel electrophoresis techniques to capillary and now next generation sequencing (NGS). Capillary was still is standard method used in analysis. However, dependent upon information needed, there are several different that can be t...

sites on the polysaccharide using simple sugars as prospective inhibitors. Although much discussion is given to this topic, a really critical evaluation of the method as it is applied would have been welcome. As if overcome by long hours of work in purifying and assaying, the members of the conference several times lapse into rather unrewarding philosophical speculation on the question of wheth...

Although analysis strategies exist for probing a diverse array of molecular properties, most of these approaches are not amenable to the study of reaction intermediates and other transient species. Separations in particular can provide detailed information on attributes not readily measured by spectroscopy but typically are performed over time scales much longer than the life span of highly uns...

bacteria play an important role in the initiation and progression of diseases (not only in disease but affect health status of individual). populations of digestive microflora in chicken gut change with age and also affected by diet, stressors, and performance enhancers as well as in disease conditions. the aim of the pre-sent study was to know bacterial makeup in chicken gut, using denaturing ...

A new fast and reliable reversed-phase high-performance liquid chromatographic method is proposed for quantifying all the major casein fractions during cheese proteolysis as an alternative to more complex methodologies. Excellent separation of caseins, para-κ-casein (ρ-κ-CN), αs1-casein (αs1-CN), αs1-I-casein (αs1-I-CN), αs2-casein (αs2-CN), β-casein (β-CN) was achieved, in terms linearity, rep...