DNA adenine methylation by DNA adenine methyltransferase (Dam) in Escherichia coli plays an important role in processes such as DNA replication initiation, gene expression regulation, and mismatch repair. In addition, E. coli strains deficient in Dam are hypersensitive to DNA-damaging agents. We used genome microarrays to compare the transcriptional profiles of E. coli strains deficient in Dam ...

T he advantages of using a rubber dam are well-known when performing operative and endodontic procedures. These benefits include the following: disolation of the operating field to keep it dry and clean; dimproved access and visibility; dpotentially improved properties of dental materials; dprotection of the patient and dentist; dimproved operating efficiency. 1 Preparing teeth for indirect res...

Large-scale dams are controversial public infrastructure projects due to the unevenly distributed benefits and losses to local regions. The central government can make redistributive fiscal transfers to attenuate the impacts and reduce the inequality among local governments, but whether large-scale dam projects are Pareto improving is still a question. Using the geographic variation of dam impa...

The Escherichia coli dam adenine-N6 methyltransferase modifies DNA at GATC sequences. It is involved in post-replicative mismatch repair, control of DNA replication and gene regulation. We show that E. coli dam acts as a functional monomer and methylates only one strand of the DNA in each binding event. The preferred way of ternary complex assembly is that the enzyme first binds to DNA and then...

We reported previously that a Salmonella enterica serovar Enteritidis dam mutant expressing a truncated Dam protein does not agglutinate in the presence of specific antibodies against O9 polysaccharide. Here we investigate the participation of Dam in lipopolysaccharide (LPS) synthesis in Salmonella. The LPS O-antigen profiles of a dam null mutant (SEDeltadam) and the Salmonella serovar Enteriti...

The Burdekin River, Queensland, Australia drains a catchment area of ~130,000 km and contributes approximately 30% of the total sediment supplied (~ 3.8 t) to the Great Barrier Reef Lagoon from all Great Barrier Reef catchments. Much of this watershed (~115,000 km) drains into the Burdekin Falls Dam, the largest dam reservoir in Queensland (1,860 GL capacity; 224 km surface area; average depth ...

Most dentists are educated in rubber dam use in dental school, but there is often disparity between what is taught for various restorative procedures and what is practiced in the private sector. It is a common, although undocumented, belief that few practicing dentists routinely use rubber dam isolation. This study repeated a survey conducted in 1985 evaluating U.S. general dentists' attitudes ...

BACKGROUND This survey study aimed at investigating the frequency of rubber dam use during root canal treatment, identifying influencing factors for not using it by Saudi general dental practitioners (GDPs) and endodontists. It also aimed at identifying measures that increase rubber dam usage. METHODS After obtaining an ethical approval, two pilot studies were conducted on staff members at Ta...