Oncolytic abilities of vaccinia virus (VACV) served as a basis for the development of various recombinants for treating cancer; however, "natural" oncolytic properties of the virus are not examined in detail. Our study was conducted to know how the genetically unmodified L-IVP strain of VACV produces its antitumor effect. Human A431 carcinoma xenografts in nude mice and murine Ehrlich carcinoma...

Purified diphtheria toxin is shown to inhibit protein synthesis in Ehrlich-Lettré ascites carcinoma cells in vitro. Protein synthesis in Ehrlich-Lettré cells is at least 10,000 times more sensitive to toxin than protein synthesis in normal mouse spleen or thymus cells. This sensitivity correlates with the observation that Ehrlich-Lettré tumors regress in mice injected with diphtheria toxin but ...

The effect of an in vivo treatment with two commonly employed drugs that are P-glycoprotein substrates, verapamil and rifampicin, on Ehrlich ascites carcinoma cells, was evaluated. Ehrlich ascites carcinoma cells were inoculated i.p. in CD-1 mice and animals were orally treated for 10 days with rifampicin (60 mg/kg/day) or verapamil (6 mg/kg/day). In the harvested cells the transcripts for mdr1...

The effects of a number of alkylating agents on the in vitro incorporation of glycine1-C ~4 into the ethanol/acetone-insoluble fraction of Ehrlich ascites carcinoma cells have been studied. With the exception of 1:6-dimethanesulfonyl-D-mannitol, all inhibit (at the concentrations tested) this incorporation in varying degrees, with little or no effect on the respiration of the cells. The process...

The proline-rich peptide (PRP-1) isolated from neurosecretory granules of the bovine neurohypophysis, produced by N.supraopticus and N.paraventricularis, has many potentially beneficial biological effects. PRP-1 been shown to have opposite effects on cell death in neurodegenerative cancer diseases. It significantly reduces staurosporine-induced apoptosis postnatal hippocampal cells, as well dox...

Egg lectin of Rana japonica, which specifically agglutinates transformed cells but does not agglutinate nontransformed cells and erythrocytes, has been isolated by gel filtration and successive ion-exchange chromatographies on diethylaminoethyl cellulose and carboxymethylcellulose columns and has been characterized as a homogeneous carbohydrate-free protein with a relative molecular weight of 1...