The importance of haploid and doubled haploid (DH) techniques for basic and applied research, as well as to improve the speed of genetic gain when applied in breeding programs, cannot be overstated. They have become routine tools in several major crop species, such as maize (Zea mays L.), wheat (Triticum aestivum L.), and barley (Hordeum vulgare L.). DH techniques in perennial ryegrass (Lolium ...

Haploid technologies are used to create homozygous lines for accelerated breeding. We aimed optimize the technology using isolated microspore culture in vitro obtain doubled haploids of carrot (Daucus carota L.).
 studied two varieties with different responsiveness embryogenesis, Altajskaya lakomka and Breeding line 17. Carrot microspores were from buds cultivated liquid nutrient media sup...

To date, all linkage maps of hexaploid oat (Avena sativa L.) have been constructed using recombinant inbred lines (RILs). Doubled haploids (DHs), however, have the advantage over RILs of their comprehensive homozygosity. DHs have been used for mapping in several cereal species, but in oats the production of large DH populations has only recently become an option. A linkage map of hexaploid oat ...

Abstract The aims of this study were to estimate the diversity doubled haploid population winter oilseed rape ( Brassica napus L.) in terms yield, yield structure and seed colour, determine correlation between studied traits their heritability, select best yellow-seeded genotypes. We 44 haploids, obtained from F 1 hybrids a cross black-seeded DH H 2 -26 line Z-114 line, as well parental forms. ...

Factors affecting reliable plant regeneration from unfertilized ovule culture of gentians (Gentiana spp.) were examined. Cold pretreatment (4°C) of flower buds enhanced or maintained production of embryo-like structure (ELS). When 43 genotypes were surveyed in two different labs, 40 of them produced ELSs ranging from 0.01 to 26.5 ELSs per flower bud. No ELSs could be obtained in three genotypes...

Microspores can be induced to develop homozygous doubled haploid plants in a single generation. In the present experiments androgenic microspores of wheat have been genetically transformed and developed into mature homozygous transgenic plants. Two different transformation techniques were investigated, one employing electroporation and the other co-cultivation with Agrobacterium tumefaciens. Di...

Doubled-haploid lines (DHs) are normally produced from F1 plants in maize (Zea mays L.). Several studies have found a low frequency of recombinants doubled haploids that could limit the selection response. Hence, an attempt was made to produce F2 generation verify whether one more round meiotic recombination lead increased genetic variability and assess response selection. The two cross-combina...