We have developed a double-sided labeling technique for detecting viral proteins or RNAs in plastic-embedded leaf tissue by immunocytochemistry or in situ hybridization, respectively, and light microscopy. The signal from the target was enhanced by double-sided labeling when compared with single-sided labeling because sections were submerged in labeling solutions with both sides accessible to a...

The fatigue cracks of rib-to-deck welded joints in an orthotropic steel deck are one the critical conundrums that restrict sustainable development bridges. Double-sided were achieved by introducing internal welding technology to overcome initial “crack-like” manufacturing defects at roots single-sided joints. Through observation macro-section joint, differences between welding, partial-penetrat...

One main improvement included in the D0 detector upgrade 1 for Run IIa is its central tracking system as shown in Fig. 1. It includes a 2 T superconducting solenoid, a Central scintillating Fiber Tracker (CFT) and the SMT. The SMT design is driven by two classes of events. Barrels and central disks cover the ∼ 25 cm RMS long luminous region for high pT central physics (|η| < 1.5). Forward disks...

[Purpose] This study aimed to investigate the effects of upper extremity-oriented intensive onesided and double-sided dribbling on the postures of basketball playing children during the period of their basic training. [Subjects] Forty male children registered for a summer basketball training course held in Isparta, Turkey, voluntarily participated in the study. [Methods] The children were forme...

Considering backlash, time-varying mesh stiffness and radial clearance of bearing, nonlinear dynamic model of gear bearing flexible shaft system is established taking into account breathing crack in shaft and tooth wear. Nonlinear dynamic equations are solved by Runge-Kutta method. Effect of backlash, crack in shaft and tooth wear faults on the nonlinear dynamic behavior of gear-shaft-bearing s...

BACKGROUND The protein product of the BRCA2 gene mediates repair of double-strand breaks in DNA. Because a number of cancer therapies exert cytotoxic effects via the initiation of double-strand breaks, cancers comprised of cells carrying BRCA2 gene mutations may be more amenable to treatment with agents that cause such breaks. METHODS We identified a human pancreatic adenocarcinoma cell line ...

After a brief summary of Tauberian conditions for ordinary sequences of numbers, we consider summability of double sequences of real or complex numbers by weighted meanmethods which are not necessarily products of related weighted mean methods in one variable. Our goal is to obtain Tauberian conditions under which convergence of a double sequence follows from its summability, where convergence ...